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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
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| Product Name | HSP27 (Phospho-Ser82) Antibody |
| Synonyms | 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser82. |
| Immunogen Range | 48-97 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 ELISA: 1:4000 |
| Specificity | HSP27 (Phospho-Ser82) Antibody detects endogenous levels of HSP27 only when phosphorylated at Ser82. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | HSPB1 |
| Database Links |
Gene ID: 3315 Swiss-Prot #: P04792 OMIM #: 602195/606595/608634 Unigene #: Hs.520973 |
| Target Modification | Phospho |
| Modification Sites | H:S82 M:S86 R:S86 |
| Application Images | |
| Image 1 |
 Western blot analysis of lysates from HUVEC cells treated with TNF 20ng/ml 30', using HSP27 (Phospho-Ser82) Antibody. The lane on the right is blocked with the phospho peptide. |
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