The antiserum was produced against synthesized peptide derived from human LIMK1 around the phosphorylation site of Thr508.
Immunogen Range
471-520
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
LIMK1 (Phospho-Thr508) Antibody detects endogenous levels of LIMK1 only when phosphorylated at Thr508.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
LIMK1
Database Links
Gene ID: 3984 Swiss-Prot #: P53667
OMIM #: 194050/601329 Unigene #: Hs.647035
Target Modification
Phospho
Modification Sites
H:T508 M:T508 R:T508
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using LIMK1 (Phospho-Thr508) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COLO205 cells, using LIMK1 (Phospho-Thr508) Antibody. The lane on the right is blocked with the phospho peptide.