Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
| ProductName | NF-kappaBp65(Phospho-Ser276)Antibody |
| Synonyms | NFKB3;nuclearfactorNF-kappa-Bp65subunit;p65;RELA;TF65;transcriptionfactorp65 |
| Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanNF-kappaBp65aroundthephosphorylationsiteofSer276. |
| ImmunogenRange | 249-298 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:20000 |
| Specificity | NF-kappaBp65(Phospho-Ser276)AntibodydetectsendogenouslevelsofNF-kappaBp65onlywhenphosphorylatedatSer276. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | RELA |
| DatabaseLinks | GeneID: 5970 Swiss-Prot#: Q04206 OMIM#: 164014 Unigene#: Hs.502875 |
| TargetModification | Phospho |
| ModificationSites | H:S276M:S276R:S276 |
| ApplicationImages |
| Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingNF-kappaBp65(Phospho-Ser276)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image2 | WesternblotanalysisoflysatesfromJurkatcells,usingNF-kappaBp65(Phospho-Ser276)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |