Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | NF-kappaBp65(Phospho-Ser276)Antibody |
Synonyms | NFKB3;nuclearfactorNF-kappa-Bp65subunit;p65;RELA;TF65;transcriptionfactorp65 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanNF-kappaBp65aroundthephosphorylationsiteofSer276. |
ImmunogenRange | 249-298 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:20000 |
Specificity | NF-kappaBp65(Phospho-Ser276)AntibodydetectsendogenouslevelsofNF-kappaBp65onlywhenphosphorylatedatSer276. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | RELA |
DatabaseLinks | GeneID: 5970 Swiss-Prot#: Q04206 OMIM#: 164014 Unigene#: Hs.502875 |
TargetModification | Phospho |
ModificationSites | H:S276M:S276R:S276 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingNF-kappaBp65(Phospho-Ser276)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromJurkatcells,usingNF-kappaBp65(Phospho-Ser276)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |