The antiserum was produced against synthesized peptide derived from human p73 around the phosphorylation site of Tyr99.
Immunogen Range
66-115
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
p73 (Phospho-Tyr99) Antibody detects endogenous levels of p73 only when phosphorylated at Tyr99.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
TP73
Database Links
Gene ID: 7161 Swiss-Prot #: O15350
OMIM #: 601990 Unigene #: Hs.697294
Target Modification
Phospho
Modification Sites
H:Y99 M:Y98
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using p73 (Phospho-Tyr99) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from K562 cells treated with Pervanadate, using p73 (Phospho-Tyr99) Antibody. The lane on the right is blocked with the phospho peptide.