The antiserum was produced against synthesized peptide derived from human CDC25A around the phosphorylation site of Ser178.
Immunogen Range
144-193
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
CDC25A (Phospho-Ser178) Antibody detects endogenous levels of CDC25A only when phosphorylated at Ser178.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDC25A
Database Links
Gene ID: 993 Swiss-Prot #: P30304
OMIM #: 116947 Unigene #: Hs.437705
Target Modification
Phospho
Modification Sites
H:S178 M:S171 R:S179
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC25A (Phospho-Ser178) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from A549 cells treated with UV 15', using CDC25A (Phospho-Ser178) Antibody. The lane on the right is blocked with the phospho peptide.