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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
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| Product Name | ITCH (Phospho-Tyr420) Antibody |
| Synonyms | AIF4; AIP4; atrophin-1-interacting protein 4; Itchy homolog E3 ubiquitin protein ligase; NAPP1; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420. |
| Immunogen Range | 386-435 |
| Clonality | Polyclonal |
| Application Dilutions | IHC: 1:50~1:100 ELISA: 1:20000 |
| Specificity | ITCH (Phospho-Tyr420) Antibody detects endogenous levels of ITCH only when phosphorylated at Tyr420. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | ITCH |
| Database Links |
Gene ID: 83737 Swiss-Prot #: Q96J02 OMIM #: 606409 Unigene #: Hs.632272 |
| Target Modification | Phospho |
| Modification Sites | H:Y420 M:Y381 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human brain, using ITCH (Phospho-Tyr420) Antibody. The picture on the right is blocked with the phospho peptide. |
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