The antiserum was produced against synthesized peptide derived from human WWOX around the phosphorylation site of Tyr33.
Immunogen Range
18-67
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
WWOX (Phospho-Tyr33) Antibody detects endogenous levels of WWOX only when phosphorylated at Tyr33.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
WWOX
Database Links
Gene ID: 51741 Swiss-Prot #: Q96KM3
OMIM #: 133239/605131 Unigene #: Hs.461453
Target Modification
Phospho
Modification Sites
H:Y33 M:Y33
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using WWOX (Phospho-Tyr33) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using WWOX (Phospho-Tyr33) Antibody. The lane on the right is blocked with the phospho peptide.