| Application | Anti-Galactocerebroside Antibody detects level of Galactocerebroside & has been published & validated for use in ELISA, IC, IH. |
| Key Applications | - ELISA
- Immunocytochemistry
- Immunohistochemistry
|
| Applications Not Recommended | - Immunohistochemistry (Paraffin)
|
| Application Notes | Immunohistochemistry: suggested starting dilution 1:10-1:50. Not suggested for use on paraffin embedded tissue sections.
Immunocytochemistry: suggested starting dilution 1:10-1:50. For staining of living cells it is recommended that the antisera be heat inactived prior to using to remove complement. Staining of living cells prior to fixation will show a typical "patching" appearance. Cells fixed prior to staining will have a more solid, "smooth" appearance.
The diluent of choice is DMEM but other buffers may be used.
The recommended fixative is 4% paraformaldehyde.
ELISA: suggested starting dilution 1:50-1:100.
Optimal working dilutions must be determined by end user. |
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