Immunogen | Syntheticpeptide. |
Host | Rabbit |
Specificity | HES-1[Hairy1].ByWesternblottheantibodyrecognizesabandat~30kDainmousebrainlysate.Anadditionalbandat~50kDamaybeseendependingonsampleandantibodyconcentrationused. |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thisproteinbelongstothebasichelix-loop-helixfamilyoftranscriptionfactors.ItisatranscriptionalrepressorofgenesthatrequireabHLHproteinfortheirtranscription.TheproteinhasaparticulartypeofbasicdomainthatcontainsahelixinterruptingproteinthatbindstotheN-boxratherthanthecanonicalE-box. |
GeneSymbol | - HES1
- HES-1
- Hes1
- HRY
- HL
- HHL
- FLJ20408
|
PurificationMethod | ImmunoAffinityPurified |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:Q14469#TranscriptionalrepressorofgenesthatrequireabHLHproteinfortheirtranscription.MayactasanegativeregulatorofmyogenesisbyinhibitingthefunctionsofMYOD1andASH1(Bysimilarity). SIZE:280aminoacids;29541Da SUBUNIT:Transcriptionrepressionrequiresformationofacomplexwithacorepressorprotein(Groucho)suchasTLE2(Bysimilarity).InteractswithSIRT1. SUBCELLULARLOCATION:Nucleus. DOMAIN:SwissProt:Q14469Hasaparticulartypeofbasicdomain(presenceofahelix-interruptingproline)thatbindstotheN-box(CACNAG),ratherthanthecanonicalE-box(CANNTG).&TheC-terminalWRPWmotifisatranscriptionalrepressiondomainnecessaryfortheinteractionwithGroucho,atranscriptionalcorepressorrecruitedtospecifictargetDNAbyHairy-relatedproteins. SIMILARITY:Contains1basichelix-loop-helix(bHLH)domain.&Contains1Orangedomain. |
MolecularWeight | ~30kDa |