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Millipore/AB15894 | Anti-Tbr2 Antibody/AB15894/100 µg

價格: ￥4308.00 市場價: 7180.00

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貨號: AB15894

品牌: Millipore

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Description
CatalogueNumber	AB15894
Description	Anti-Tbr2Antibody
AlternateNames	Eomesoderminhomolog T-boxbrainprotein2 T-brain-2 TBR-2 Tbr2
BackgroundInformation	Eomesoderminhomolog(UniProtO54839;alsoknownasT-boxbrainprotein2,T-brain-2,TBR-2)isencodedbytheEomes(alsoknownasTbr2,C77258)gene(GeneID13813)inmurinespecies.Tbr2isamemberofaconservedproteinfamilythatsharesacommonDNA-bindingdomain,theT-box.T-boxgenesencodetranscriptionfactorsinvolvedintheregulationofdevelopmentalprocesses.Trb2hasbeenshowntoplayaroleinthedevelopmentoftheneocortexandfunctionsinthedifferentiationpathwayofrADIalgliatopost-mitoticprojectionneuron.

ProductInformation
Format	AffinityPurified
Control	E13-14mousebrainlysate
Presentation	Purifiedchickenpolyclonalantibodyinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat2-8°Cfromdateofreceipt.

Applications
Application	ThisAnti-Tbr2antibodyisvalidatedforuseinWesternBlottingandImmunohistochemistry(Paraffin)forthedetectionofTbr2.
KeyApplications	WesternBlotting Immunohistochemistry
ApplicationNotes	ImmunohistochemistryAnalysis:A1:1,000dilutionfromarepresentativelotdetectedTbr2inmousecerebralcortexandmousecerebellumtissue. WesternBlot(SNAPID)Analysis:0.5μg/mlfromapreviouslotdetectedTbr2in10μgofE13-14mousebrainlysate. ImmunohistochemistryAnalysis:1:100dilutionfromapreviouslotdetectedTbr2inmouseandhumanbrain.

BIOLOGicalInformation
Immunogen	KLH-conjugatedlinearpeptidecorrespondingtomouseTbr2.
Epitope	TranscriptionActivationDomain
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Chicken
Specificity	ExpectedtoreactwithbothsplicedisoformsofmurineTbr2.
Isotype	IgY
SpeciesReactivity	Mouse Human Rat
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NP_034266
GeneSymbol	Eomes Tbr2
PurificationMethod	AffinityPurfied
UniProtNumber	O54839
UniProtSummary	FUNCTION:Functionsasatranscriptionalactivatorplayingacrucialroleduringdevelopment.Functionsintrophoblastdifferentiationandlateringastrulation,regulatingbothmesodermdelaminationandendodermspecification.PlaysaroleinbraindevelopmentbeingrequiredforthespecificationandtheproliferationoftheintermediateProgenitorcellsandtheirprogenyinthecerebralcortex.AlsoinvolvedinthedifferentiationofCD8+T-cellsduringimmuneresponseregulatingtheexpressionoflyticeffectorgenes. SUBCELLULARLOCATION:NucleusProbable. TISSUESPECIFICTY:ExpressedinCD8+T-cells. DEVELOPMENTALSTAGE:Originallyexpressedinthetrophoectodermoftheblastocystandlaterintheextra-embryonicectodermoftheearlypost-implantationembryo.Intheembryoproper,expressedintheposteriorpartoftheepIBLast.Duringgastrulation,extendsdistallyintotheprimitivestreakandnascentmesoderm.Alsoexpressedinthedevelopingforebrainandtheolfactorylobes.ExpressedatE12.5andE14.5intheforebrain. INDUCTION:Up-regulatedinCD8+T-cellsuponactivation. DISRUPTIONPHENOTYPE:Embryoniclethalduetoperi-implantationdefects.Mutantembryosarrestsoonafterimplantationandfailtoformorganizedembryonicorextraembryonicstructures.Conditionalmutants,withexpressionabrogatedintheinnercellmassofembryosfromearlyimplantationstagesonward,displaygastrulationdefects. SEQUENCESIMLARITIES:Contains1T-boxDNA-bindingdomain.
MolecularWeight	~85/95kDaobserved.Targetbandsappearlargerthanthecalculatedmolecularsizeof74.8kDa(isoform1)and72.7kDa(isoform2).

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Millipore/AB15894 | Anti-Tbr2 Antibody/AB15894/100 µg

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