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Millipore/AB1875 | Anti-VEGF Receptor-3 Antibody/AB1875/100 µg

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貨號: AB1875

品牌: Millipore

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Description
CatalogueNumber	AB1875
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-VEGFReceptor-3Antibody
AlternateNames	FLT-4

ProductInformation
Format	Purified
Control	POSITIVECONTROL:humanbreastcancercelllineMCF7
Presentation	Protein-ApurifiedIgGfractioninPBS,pH7.4,with0.1%BSAand0.0015%sodiumazideasapreservative.

StorageandShippingInformation
StorageConditions	Maintainat-20°Cinundilutedaliquotsforupto12monthsafterdateofreceipt.Avoidrepeatedfreeze/thawcycles. Duringshipment,smallvolumesofantibodywilloccasionallybecomeentrappedinthesealoftheproductvial.Forantibodieswithvolumesof200μlorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialonatabletopcentrifugetodislodgeanyliquidinthecontainer"scap.

Applications
Application	DetectVEGFReceptor-3usingthisAnti-VEGFReceptor-3AntibodyvalidatedforuseinIC,IH(P)&WB.
KeyApplications	Immunocytochemistry Immunohistochemistry(Paraffin) WesternBlotting
ApplicationNotes	Immunohistochemistryonformalinfixedparaffinsections:1:100-1:400.Hightemperatureantigenretrievalin10mMcitratebuffer(pH6.0)isstronglyrecommended.Blockingwith2%normalserumplus1%BSAinPBSissuggested.PrimaryantibodyshouldbedilutedinPBSwith0.1%BSAonly.TheuseofTritonX-100orexcessblockingcompoundsisnotrecommended. WesternBlot:antibodyrecognizesthreebandsmigratingatapproximately198kDa,94kDaand47kDacorrespondingtothelongandshortformsofthereceptorplusapossIBLebreakdownproduct,respectively.Variationinbandpositionisexpectedduetosignificantvariationinexpressionandglycosylationinvarioustissuesandcelllines.Membraneextractpreparationsandtheliberaluseofproteinaseinhibitorsisstronglyrecommended. Immunocytochemistry:MCF7,SW480andMDA-MBcelllinesarepositivecontrols. Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Immunogen	Raisedagainsta12aminoacidsequencecorrespondingtotheN-terminusofthehumanvascularendothelialgrowthfactorreceptor3[VEGFR3/FLT4].
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rabbit
Specificity	AB1875isspecificfortheN-terminusofVEGFReceptor3[FLT4]and,thus,itwillrecognizeboththelongandshortformsofthereceptor.Stainingofformalinfixedparaffinembeddedhumantissuesectionsispredominantlylocalizedinthecytoplasm.
SpeciesReactivity	Human
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_002020.3 NM_182925.3
GeneSymbol	FLT4 PCL VEGFR-3 FLT41 VEGFR3 EC2.7.10.1
UniProtNumber	P35916
UniProtSummary	FUNCTION:SwissProt:P35916#ReceptorforVEGFC.Hasatyrosine-proteinkinaseactivity. SIZE:1298aminoacids;145599Da SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. TISSUESPECIFICITY:Placenta,lung,heart,andkidney,doesnotseemtobeexpressedinpancreasandbrain. DISEASE:SwissProt:P35916#DefectsinFLT4arethecauseofhereditarylymphedemaI[MIM:153100];alsoknownasNonne-MilroylymphedemaorMilroydisease.Hereditarylymphedemaisachronicdisablingconditionwhichresultsinswellingoftheextremitiesduetoalteredlymphaticflow.Patientswithlymphedemasufferfromrecurrentlocalinfectionsandphysicalimpairment.HereditarylymphedemaIshowsautosomaldominantinheritanceandischaracterizedbyonsetusuallyatbirth.&DefectsinFLT4arefoundinjuvenilehemangioma.Juvenilehemangiomasarethemostcommontumorsofinfancy,occurringasmanyas10/%ofallbirths.Thesebenignvascularlesionsenlargerapidlyduringthefirstyearoflifebyhyperplasiaofendothelialcellsandattendantpericytes,andthenspontaneouslyinvoluteoveraperiodofyears,leavingloosefibrofattytissue. SIMILARITY:SwissProt:P35916##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.CSF-1/PDGFreceptorsubfamily.&Contains7Ig-likeC2-type(immunoglobulin-like)domains.&Contains1proteinkinasedomain.

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Millipore/AB1875 | Anti-VEGF Receptor-3 Antibody/AB1875/100 µg

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