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Millipore/AB3623 | Anti-Caspase 3 Antibody, active (cleaved) form/AB3623/50 µg

價格: ￥4068.00 市場價: 6780.00

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貨號: AB3623

品牌: Millipore

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Description
CatalogueNumber	AB3623
Replaces	04-440;04-1088
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-Caspase3Antibody,active(cleaved)form
AlternateNames	Apopain YamaProtein CASP3 SCA-1 procaspase3
BackgroundInformation	Caspase-3(CPP32,Apopain)isthemostextensivelystudiedapoptoticprotein.Caspase-3issynthesizedasaninactiveproenzyme(32kDa)thatisprocessedincellsundergoingapoptosisbyself-proteolysisand/orcleavagebyanotherupstreamprotease.Theprocessedformofcaspase-3consistsoflarge(17kDa)andsmall(12kDa)subunitswhichassociatetoformanactiveenzyme.Theactivecaspase-3proteolyticallycleavesandactivatesothercaspases,aswellasrelevanttargetsinthecellssuchasPARPandDFF.

ProductInformation
Format	AffinityPurified
Control	Tonsilandappendixtissue.JurkatcellsinducedforapoptosiswithrecombinantsolubleTRAILandstaurosporinetreatedmouse3T3celllysatesSkin/Tumor,Embryo.
Presentation	PurifiedrabbitpolyclonalinbuffercontainingPBS,containing50%glycerol,1%BSAand0.02%thimerosal.

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20oCfromdateofreceipt.

Applications
Application	Anti-Caspase3Antibody,active(cleaved)formdetectslevelofCaspase3andhasbeenpublishedandvalidatedforuseinImmunofluorescence(IF),Immunohistochemistry(IHC),andWesternBlot(WB).
KeyApplications	Immunofluorescence Immunohistochemistry Immunohistochemistry(Paraffin) WesternBlotting
ApplicationNotes	WesternBlotAnalysis: 1:100-1:200(0.5-4μg/mL)dilutionofactiveapoptoticcellsweusedtodetectCaspase-3. Notewhenusingtissue,levelsofactivecaspase3canbelowdetectablelevels.UseCC119(humancaspase3)incubated30minuteswith1mMATP(foractivation)asapositivecontrolonwesterns.Alternatively,neuronalculturepreparationsincubatedwithstaurosporine(0.5μM,10-18hr)typicallygiveastrongcaspase3signal. Immunohistochemistry: 1:10dilution(10-20μg/mL),AB3623hasreactedsuccessfullyin4%PFAfixed,paraffinembeddedtissuesmousetissues. Immunohistochemistry(paraffin): OptimalStainingofCaspase-3MonoclonalAntibody:MouseSmallIntestine

BIOLOGicalInformation
Immunogen	Cleavagespecificsyntheticpeptidesequence(proprietary).
Epitope	active(cleaved)form
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rabbit
Specificity	AB3623detectsonlythecleavedp17fragmentanddoesnotdetecttheprecursorformincellsundergoingapoptosis.
SpeciesReactivity	Human Mouse Rat
SpeciesReactivityNote	Human,Mouse,Rat.Reactivitywithotherspecieshasnotbeendetermined.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_004346.3
EntrezGeneSummary	Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein.
GeneSymbol	CASP3 CPP-32 Yama CASP-3 CPP32B SCA-1 CPP32 EC3.4.22.56[Contains:Caspase-3subunitp17Caspase-3subunitp12].
PurificationMethod	ImmunoAffinityPurified
UniProtNumber	P42574
UniProtSummary	FUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin. SIZE:277aminoacids;31608Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit. SUBCELLULARLOCATION:Cytoplasm. TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem. PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa. SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family.
MolecularWeight	17kDa

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Millipore/AB3623 | Anti-Caspase 3 Antibody, active (cleaved) form/AB3623/50 µg

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