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Millipore/AB3756 | Anti-Rad51 Antibody/AB3756/100 µg

價格: ￥4068.00 市場價: 6780.00

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貨號: AB3756

品牌: Millipore

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Description
CatalogueNumber	AB3756
Replaces	MABE515
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-Rad51Antibody

ProductInformation
Format	AffinityPurified
Presentation	Affinitypurifiedimmunoglobulin.Precipitatedantibodyinasolutionof50%saturatedammoniumsulfateandPBScontainingnopreservatives.

StorageandShippingInformation
StorageConditions	Maintainunopenedvialat-70Cforupto6months.Avoidrepeatedfreeze/thawcycles. Preparation&Use:Toreconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammoniumsulfate/PBSsolution:10μLofresidualammoniumsulfatesolutionwillnoteffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur. ResuspendtheantibodypelletinanysuitableBIOLOGicalbuffer,standardPBSorTBS(pH7.3-7.5)aretypical.Volumesrequiredarenotcriticalbutitissuggestedthatthefinalantibodyconcentrationbebetween0.1mg/mLand1.0mg/mL.Forexample,toachievea1mg/mLconcentrationwith50μgofprecipitatedantibody,theamountofbufferneededwouldbe50μL. Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25C°priortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses. Therehydratedantibodysolutionscanbestoredundilutedat2-8C°for2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8C°.Forstorageat-20C°,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.Forfreezing,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70C°forupto6months.Avoidrepeatedfreeze/thawcycles.

StorageandShippingInformation

StorageConditions

Maintainunopenedvialat-70Cforupto6months.Avoidrepeatedfreeze/thawcycles.

Preparation&Use:Toreconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammoniumsulfate/PBSsolution:10μLofresidualammoniumsulfatesolutionwillnoteffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur.

ResuspendtheantibodypelletinanysuitableBIOLOGicalbuffer,standardPBSorTBS(pH7.3-7.5)aretypical.Volumesrequiredarenotcriticalbutitissuggestedthatthefinalantibodyconcentrationbebetween0.1mg/mLand1.0mg/mL.Forexample,toachievea1mg/mLconcentrationwith50μgofprecipitatedantibody,theamountofbufferneededwouldbe50μL.

Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25C°priortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses.

Therehydratedantibodysolutionscanbestoredundilutedat2-8C°for2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8C°.Forstorageat-20C°,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.Forfreezing,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70C°forupto6months.Avoidrepeatedfreeze/thawcycles.

Applications
Application	DetectRad51withAnti-Rad51Antibody(RabbitPolyclonalAntibody),thathasbeenshowntoworkinWB,ICC&IHC.
KeyApplications	WesternBlotting Immunocytochemistry Immunohistochemistry
ApplicationNotes	Westernblot:1:200-1:1,000usingECL. Immunohistochemistry:1:200-1:1,000. Immunocytochemistry:1:200-1:1,000. Optimalworkingdilutionsmustbedeterminedbytheenduser. SUGGESTEDPROTOCOLFORIMMUNOHISTOCHEMISTRY 1.Dissecttissuesandfreezeondryice 2.Cutonacryostat-10umslices 3.Dryslidesatroomtemperaturefor30-90min 4.FixwithcoldAcetone/Methanol(50/50)2min-theneitherprocessfurtherorstoreat-20°C.Alternativeisfixationwith4%PFAandtreatmentwithatrypsinsolution.(0.05%trypsin,incubation10minutesat37°C,followedby3washeswithPBS) 5.Letairdry 6.PBS5min 7.Incubatein50mMammoniumchloride30min 8.PBS5min 9.Blockingserum30-45min 10.Primaryantibody90min-dilution1:100-1:600 11.Wash3timesfor5min 12.Secondaryfluorescentconjug.antibody30min 13.Wash3timesfor5minandmount

BiologicalInformation
Immunogen	Syntheticpeptide,aminoacids25-41ofRAD51.
Host	Rabbit
Specificity	RecognizesRAD51.Themolecularweightoftheproteinis36,963Daltons.
SpeciesReactivity	Human Mouse
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_002875.3 NM_133487.2
EntrezGeneSummary	TheproteinencodedbythisgeneisamemberoftheRAD51proteinfamily.RAD51familymembersarehighlysimilartobacterialRecAandSaccharomycescerevisiaeRad51,andareknowntobeinvolvedinthehomologousrecombinationandrepairofDNA.ThisproteincaninteractwiththessDNA-bindingproteinRPAandRAD52,anditisthoughttoplayrolesinhomologouspairingandstrandtransferofDNA.ThisproteinisalsofoundtointeractwithBRCA1andBRCA2,whichmaybeimportantforthecellularresponsetoDNAdamage.BRCA2isshowntoregulateboththeintracellularlocalizationandDNA-bindingABIlityofthisprotein.LossofthesecontrolsfollowingBRCA2inactivationmaybeakeyeventleADIngtogenomicinstabilityandtumOrigenesis.Twoalternativelysplicedtranscriptvariantsofthisgene,whichencodedistinctproteins,havebeenreported.TranscriptvariantsutilizingalternativepolyAsignalsexist.
GeneSymbol	RAD51 RAD51L3 HsT16930 BRCC5 hRAD51 HRAD51 RECA HsRad51 RAD51D HsRAD51 RAD51A
UniProtNumber	Q06609
UniProtSummary	FUNCTION:SwissProt:Q06609#MayparticipateinacommonDNAdamageresponsepathwayassociatedwiththeactivationofhomologousrecombinationanddouble-strandbreakrepair.BindstosingleanddoublestrandedDNAandexhibitsDNA-dependentATPaseactivity.UnderwindsduplexDNAandformshelicalnucleoproteinfilaments. SIZE:339aminoacids;36966Da SUBUNIT:InteractswithBRCA1,BRCA2andeitherdirectlyorindirectlywithp53.InteractswithXRCC3,RAD54LandRAD54B.PartofacomplexwithRAD51CandRAD51B.InteractswithRAD51AP1.InteractswithCHEK1/CHK1,andthismayrequirepriorphosphorylationofCHEK1. SUBCELLULARLOCATION:Nucleus.Note=ColocalizeswithRAD51AP1tomultiplenuclearfociuponinductionofDNAdamage. TISSUESPECIFICITY:Highlyexpressedintestisandthymus,followedbysmallintestine,placenta,colon,pancreasandovary.Weaklyexpressedinbreast. PTM:Phosphorylated.PhosphorylationofThr-309byCHEK1/CHK1mayenhanceassociationwithchromatinatsitesofDNAdamageandpromoteDNArepairbyhomologousrecombination.DISEASE:SwissProt:Q06609#DefectsinRAD51areassociatedwithbreastcancer(BC)[MIM:114480]. SIMILARITY:SwissProt:Q06609##BelongstotherecAfamily.RAD51subfamily.&Contains1HhHdomain.

PhysicochemicalInformation

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Millipore/AB3756 | Anti-Rad51 Antibody/AB3756/100 µg

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