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Millipore/AB5935 | Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40/AB5935/100 µL

價格: ￥4308.00 市場價: 7180.00

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貨號: AB5935

品牌: Millipore

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Description
CatalogueNumber	AB5935
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-TyrosineHydroxylaseAntibody,phosphoSer40
AlternateNames	TyrosineMonooxygenase
BackgroundInformation	Tyrosinehydroxylaseplaysanimportantroleinthephysiologyofadrenergicneurons.Itisthefirstandrate-limitingenzymeinvolvedinthebiosynthesisofthecatecholaminesDopamineandNorepinephrinefromtyrosine.THis,therefore,ausefulMarkerfordopaminergicandnoradrenergicneurons.TheenzymaticactivityofTHrequiresferrousionsascofactorsandisbelievedtoberegulatedbyphosphorylation.AtleastfourisoformsofhumanTHhavebeenidentifiedwhichresultfromalternativesplicing.

ProductInformation
Format	AffinityPurified
Control	POSITIVECONTROL:brain(sympatheticnerveterminals)andadrenalglands.
Presentation	Purifiedrabbitpolyclonalliquidinbuffercontaining10mMHepes,pH7.5,150mMNaCl,100μg/mLBSA,and50%glycerol.

StorageandShippingInformation
StorageConditions	Stablefor6monthsat-20°Cinundilutedfromdateofreceipt.

Applications
Application	Anti-TyrosineHydroxylaseAntibody,phosphoSer40detectslevelofTyrosineHydroxylase&hasbeenpublished&validatedforuseinIC,IH&WB.
KeyApplications	Immunocytochemistry Immunohistochemistry WesternBlotting
ApplicationNotes	Immunohistochemistry: A1:1,000dilutionofapreviouslotwasusedinIH. Immunocytochemistry: A1:1,000dilutionofapreviouslotwasusedinIC. Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Immunogen	SyntheticpeptidefromthephosphoSer40ofratTyrosineHydroxylase.
Epitope	phospho-Ser40
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rabbit
Specificity	TyrosineHydroxylase,phosphoSer40.Theantibodyrecognizesaproteinof60kDacorrespondingtoTHphosphorylatedatSer40.Dependingonbrainregionbeingstudied,proteinloadsanddetectionmethodsusedsomehighermolecularweightbandsmaybedetected.
SpeciesReactivity	Rat Mouse Human Avian
SpeciesReactivityNote	Ratandmouse.Itisexpectedthattheantibodywillalsoreactwithhuman,porcine,quailandnon-humanprimatesduetosequencehomology(100%).Otherspecieshavenotbeentested.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_000360.3
EntrezGeneSummary	Tyrosinehydroxylaseisinvolvedintheconversionoftyrosinetodopamine.Astherate-limitingenzymeinthesynthesisofcatecholamines,tyrosinehydroxylasehasakeyroleinthephysiologyofadrenergicneurons.
GeneSymbol	TH TYH EC1.14.16.2
Modifications	Phosphorylation
PurificationMethod	ImmunoAffinityPurified
UniProtNumber	P07101
UniProtSummary	FUNCTION:SwissProt:P07101#Playsanimportantroleinthephysiologyofadrenergicneurons. COFACTOR:Fe(2+)ion. SIZE:528aminoacids;58524Da TISSUESPECIFICITY:Mainlyexpressedinthebrainandadrenalglands. DISEASE:SwissProt:P07101#DefectsinTHarethecauseofautosomalrecessiveSegawasyndrome[MIM:605407];alsoknownasDOPA-responsivedystonia.Typically,itbeginsinchildhoodoradolescencewithprogressivedifficultyinwalkingand,insomecases,spasticity.Somecasespresentwithparkinsoniansymptomsininfancyandarereferredtoasautosomalrecessiveinfantileparkinsonism. SIMILARITY:SwissProt:P07101##Belongstothebiopterin-dependentaromaticaminoacidhydroxylasefamily.
MolecularWeight	60kDa

PhysicochemicalInformation

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Millipore/AB5935 | Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40/AB5935/100 µL

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