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Millipore/AB5995 | Anti-Olig3 Antibody/AB5995/100 µg

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貨號: AB5995

品牌: Millipore

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Description
CatalogueNumber	AB5995
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-Olig3Antibody

ProductInformation
Format	AffinityPurified
Presentation	Affinitypurifiedimmunoglobulin.Precipitatedantibodyinasolutionof50%saturatedammoniumsulfateandPBScontainingnopreservatives.

StorageandShippingInformation
StorageConditions	Maintainunopenedvialat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles. PREPARATIONANDUSE: Toreconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammoniumsulfate/PBSsolution:10μLofresidualammoniumsulfatesolutionwillnotaffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur. ResuspendtheantibodypelletinasuitableBIOLOGicalbuffersuchasPBSorTBS(pH7.3-7.5)toafinalconcentrationof1.0mg/mL.Forexample,toachievea1.0mg/mLconcentrationwith50μgofprecipitatedantibody,theamountofbufferneededwouldbe50μL. Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25°Cpriortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses. Therehydratedantibodysolutionscanbestoredundilutedat2-8°Cfor2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8°C. Forstorageat-20°C,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality. Forlong-termstorageat-70°C,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.

StorageandShippingInformation

StorageConditions

Maintainunopenedvialat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.

PREPARATIONANDUSE:

Toreconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammoniumsulfate/PBSsolution:10μLofresidualammoniumsulfatesolutionwillnotaffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur.

ResuspendtheantibodypelletinasuitableBIOLOGicalbuffersuchasPBSorTBS(pH7.3-7.5)toafinalconcentrationof1.0mg/mL.Forexample,toachievea1.0mg/mLconcentrationwith50μgofprecipitatedantibody,theamountofbufferneededwouldbe50μL.

Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25°Cpriortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses.

Therehydratedantibodysolutionscanbestoredundilutedat2-8°Cfor2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8°C.

Forstorageat-20°C,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.

Forlong-termstorageat-70°C,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.

Applications
Application	Anti-Olig3AntibodydetectslevelofOlig3&hasbeenpublished&validatedforuseinWB,IC,IH.
KeyApplications	WesternBlotting Immunocytochemistry Immunohistochemistry
ApplicationNotes	Westernblot:1:200-1:1,000usingECL. Immunohistochemistry:1:200-1:1,000. Immunocytochemistry:1:200-1:1,000. Optimalworkingdilutionsmustbedeterminedbytheenduser.

BiologicalInformation
Immunogen	Syntheticpeptide,aminoacids53-66ofOlig3.
Host	Rabbit
Specificity	RecognizesOlig3.Themolecularweightoftheproteinis27,909Daltons.
SpeciesReactivity	Human Mouse
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_175747.2
GeneSymbol	OLIG3 BHLHB7 bHLHB7 Oligo3 Bhlhb7
UniProtNumber	Q7RTU3
UniProtSummary	FUNCTION:SwissProt:Q7RTU3#MaydeterminethedistinctspecificationprogramofclassAneuronsinthedorsalpartofthespinalcordandsuppressspecificationofclassBneurons. SIZE:272aminoacids;29358Da SUBCELLULARLOCATION:Nucleus(Bysimilarity). SIMILARITY:SwissProt:Q7RTU3##Contains1basichelix-loop-helix(bHLH)domain.

PhysicochemicalInformation

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MaterialsInformation

MaterialsInformation

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Millipore/AB5995 | Anti-Olig3 Antibody/AB5995/100 µg

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