當前位置： Millipore >>> Millipore/AG400 | BLACK-GOLD? II Stain/AG400/150 mg

Millipore/AG400 | BLACK-GOLD? II Stain/AG400/150 mg

價格: ￥4308.00 市場價: 7180.00

打印產品信息

貨號: AG400

品牌: Millipore

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即購買加入購物車

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

Description
CatalogueNumber	AG400
Replaces	AG390
TradeName	BLACK-GOLD
Description	BLACK-GOLD?IIStain
Overview	Black-Gold?IIstainisanaurohalophosphatecomplexwhichstainsspecificallyformyelinwithinthecentralnervoussystem.Black-GoldIIstainingprovideshighresolution,highcontrast,ashorthistochemicalprocessingtime,andhighreproducibility.Black-GoldIIstainisanewandimprovedversionofitspredecessor,Black-Goldstain(discontinued).TheadvantagesofMillipore’sBlack-GoldIIoverBlack-GoldIstainarethatitismorereADIlysoluble,canbeutilizedatahigherconcentration,producesamoreuniformandconsistentstaining,takeslesstimetostain,anddoesnotrequirepost-stainingintensificationsteps. TheuseofBlack-GoldIIstainistailoredtostudiesusingformalin-fixed,non-solventprocessedtissue.Thetechniquestainslargemyelinatedtractsdarkred-brown,whiletheindividualmyelinatedfibersappearblack.Thisnoveltracercanbeusedtolocalizebothnormalandpathologicalmyelin.Black-GoldIIstaincandemonstrateandcharacterizespecificmyelinchangesassociatedwithexposuretodiverseneurotoxicantsincludingkainicacid,methamphetamine,acrylamide,domoicacid,3-nitropropionicacid,Fluoro-Gold?tracerandisoniazid. BlackGoldIIPurity:Nodetectableamountofuncomplexedgoldwasfound. BlackGoldIIIllumination:Brightfieldordarkfield. BlackGoldIISolubility:Freelysolubleinwater,saline,ordiluteacids.

ProductInformation
Components	Black-GoldII:150mgdrypowder,yellowincolor.
Presentation	SeeComponents

StorageandShippingInformation
StorageConditions	Thekitcanbestoredatroomtemperature.Ideally,theBlack-GoldIIpowdershouldbekeptinadesiccator,becauseofitshygroscopicnature.Thedrypowder,ifunopenedandstoredproperly,isusableforuptooneyearfromdateofreceipt.OnceresUSPendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months. TOXICITY:Althoughthecompoundappearstobeoflowtoxicity,ithasnotbeenextensivelyevaluatedandthereforeroutinelaboratorycautionshouldbeexercised.Notintendedforhumanconsumption.

StorageandShippingInformation

StorageConditions

Thekitcanbestoredatroomtemperature.Ideally,theBlack-GoldIIpowdershouldbekeptinadesiccator,becauseofitshygroscopicnature.Thedrypowder,ifunopenedandstoredproperly,isusableforuptooneyearfromdateofreceipt.OnceresUSPendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months.

TOXICITY:Althoughthecompoundappearstobeoflowtoxicity,ithasnotbeenextensivelyevaluatedandthereforeroutinelaboratorycautionshouldbeexercised.Notintendedforhumanconsumption.

Applications
KeyApplications	Immunohistochemistry
ApplicationNotes	TissueFixation: Tissuecanbefixedwitheitherformalinor4%PFA.Typicallyanimalsareperfusedwithneutralphosphatebuffered10%formalin(or4%formaldehyde)viatheascendingaorta.Thebrainsarethenremovedandfixedovernightinthesamefixativesolution.Longerfixationtimesaregenerallynotrecommended.Forcryosections,thebrainsmaybeprotectedthroughtreatmentwithsucrosesolution(20-30percent).Subsequentlyfrozenbraintissueiscutonafreezingslidingmicrotome.Note:Black-GoldIIwillnotstainparaffinembeddedorunfixedtissue. Sectioning: Eitherfrozenorfixed,non-frozenvibratomesectionscanbeusedandshouldbecutatathicknessof20-40μm.Thesectionsarethentypicallymountedonpositivelychargedslides.Thesectionscanalsobestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides. Solutions: Black-GoldIIpowdershouldberesuspendedwithsalinesolution(0.9%NaCl)toafinalconcentrationof0.3%.Simplyaddthe150mgofBlack-GoldIIpowderinto50mlsof0.9%NaClsolutionandmixtoresuspend.OnceresuspendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months. Staining: 1)Formalin-fixed,non-embeddedtissuesectionsarefirstmountedfromdistilledwaterontogelatin-coatedslidesandthenairdriedat50-60oCforatleast?houronaslidewarmer. 2)TissuesectionsarethenrehydratedindistilledMilli-Q?waterforabout2minutes. 3)Theslidesarethentransferredtoa0.3%Black-GoldIIsolutiondissolvedin0.9%salinevehicleandheatedto60-65oC.Afterabout12minutes,microscopicmonitoringoftheextentofthelabelingisrecommended.Thismonitoringshouldberepeatedevery2-3minutesuntilthedesireddegreeofmyelinimpregnationisobserved(seebelow). 4)Rinsetheslidesforabout2minindistilledMilli-Qwater. 5)Transfertheslidestoa1%sodiumthiosulfatesolutionfor3minutes. 6)Rinsetheslideswitheitherthree,fiveminchangesoftapwateror15minsofrunningtapwater. Post-stainingsteps: 7)Dehydratesectionseitherviagraduatedalcoholicsolutionsorbyairdryingonaslidewarmer. 8)Immersesectionsfor1-2mininxyleneorhistoclearandthencoverslipwithanon-polarmountingmedia.

Applications

KeyApplications

Immunohistochemistry

ApplicationNotes

TissueFixation:
Tissuecanbefixedwitheitherformalinor4%PFA.Typicallyanimalsareperfusedwithneutralphosphatebuffered10%formalin(or4%formaldehyde)viatheascendingaorta.Thebrainsarethenremovedandfixedovernightinthesamefixativesolution.Longerfixationtimesaregenerallynotrecommended.Forcryosections,thebrainsmaybeprotectedthroughtreatmentwithsucrosesolution(20-30percent).Subsequentlyfrozenbraintissueiscutonafreezingslidingmicrotome.Note:Black-GoldIIwillnotstainparaffinembeddedorunfixedtissue.

Sectioning:
Eitherfrozenorfixed,non-frozenvibratomesectionscanbeusedandshouldbecutatathicknessof20-40μm.Thesectionsarethentypicallymountedonpositivelychargedslides.Thesectionscanalsobestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides.

Solutions:
Black-GoldIIpowdershouldberesuspendedwithsalinesolution(0.9%NaCl)toafinalconcentrationof0.3%.Simplyaddthe150mgofBlack-GoldIIpowderinto50mlsof0.9%NaClsolutionandmixtoresuspend.OnceresuspendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months.

Staining:
1)Formalin-fixed,non-embeddedtissuesectionsarefirstmountedfromdistilledwaterontogelatin-coatedslidesandthenairdriedat50-60oCforatleast?houronaslidewarmer.
2)TissuesectionsarethenrehydratedindistilledMilli-Q?waterforabout2minutes.
3)Theslidesarethentransferredtoa0.3%Black-GoldIIsolutiondissolvedin0.9%salinevehicleandheatedto60-65oC.Afterabout12minutes,microscopicmonitoringoftheextentofthelabelingisrecommended.Thismonitoringshouldberepeatedevery2-3minutesuntilthedesireddegreeofmyelinimpregnationisobserved(seebelow).
4)Rinsetheslidesforabout2minindistilledMilli-Qwater.
5)Transfertheslidestoa1%sodiumthiosulfatesolutionfor3minutes.
6)Rinsetheslideswitheitherthree,fiveminchangesoftapwateror15minsofrunningtapwater.

Post-stainingsteps:
7)Dehydratesectionseitherviagraduatedalcoholicsolutionsorbyairdryingonaslidewarmer.
8)Immersesectionsfor1-2mininxyleneorhistoclearandthencoverslipwithanon-polarmountingmedia.

BIOLOGicalInformation

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Parafilm M封口膜/PM996/4x125/盒
￥0.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥850.00
ThermoFisherQ32856Qubit? Assay Tubes現貨
￥590.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00

Millipore/AG400 | BLACK-GOLD? II Stain/AG400/150 mg

客戶服務

何為188

關注188微信公眾號