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Millipore/APT131 | Caspase 3 Colorimetric Activity Assay Kit, DEVD/APT131/25 assays

價格: ￥3300.00 市場價: 5500.00

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貨號: APT131

品牌: Millipore

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Description
CatalogueNumber	APT131
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Caspase3ColorimetricActivityAssayKit,DEVD
Overview	ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.Chemicon"sCaspase-3ColorimetricActivityAssayKitsprovideasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceDEVD.Theassayisbasedonspectophotometricdetectionofthechromophorep-nitroaniline(pNA)aftercleavagefromthelabeledsubstrateDEVD-pNA.ThefreepNAcanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat405nm.ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseincaspase-3activity.
AlternateNames	CPP32
MaterialsRequiredbutNotDelivered	·Microcentrifugeand1.5mLMicrocentrifugetubes ·37°CWaterbathorIncubator ·SpectrophotometerorMicroplateReader ·96wellMicrotiterPlate

ProductInformation
Components	5XCellLysisBuffer(PartNo.90065):5mL 5XAssayBuffer(PartNo.90066):10mL Caspase-3Substrate(Ac-DEVD-pNA)(PartNo.90079):250μLof3mg/mLsolution Caspase-3Inhibitor(Ac-DEVD-CHO)(PartNo.90067):50μLof100μM(0.05mg/mL)inDMSO pNAStandard(PartNo.90085):250μLof10mMinDMSO
Detectionmethod	Chromogenic

StorageandShippingInformation
StorageConditions	Storekitmaterialsat-20°Cuptotheirexpirationdate. SpecialPrecautions: ·Afterthawingreagents,useimmediatelyoraliquotandfreezeat-20°Cforlongerstorage.Avoidrepeatedfreeze/thawcycles. ·TheCaspase-3substrateandpNAstandardareespeciallylightsensitive.Maintainthesereagentsinamberorcoveredcontainers.

Applications
Application	Caspase-3ColorimetricActivityAssayKitsprovideasimple&convenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceDEVD.
KeyApplications	ActivityAssay

BIOLOGicalInformation
SpeciesReactivity	Mammals
EntrezGeneNumber	NM_004346.3 NM_032991.2
EntrezGeneSummary	Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein.
GeneSymbol	CASP3 apopain procaspase3 CPP-32 Apopain Yama CASP-3 CPP32B SCA-1 CPP32 EC3.4.22.56[Contains:Caspase-3subunitp17 Caspase-3subunitp12].
UniProtNumber	P42574
UniProtSummary	FUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin. SIZE:277aminoacids;31608Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit. SUBCELLULARLOCATION:Cytoplasm. TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem. PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa. SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family.

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Millipore/APT131 | Caspase 3 Colorimetric Activity Assay Kit, DEVD/APT131/25 assays

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