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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | POLR2A Antibody |
| Synonyms | DNA-directed RNA polymerase II largest subunit; RNA pol II largest subunit; RNA polymerase II 220 kd subunit; RPB1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human POLR2A. |
| Immunogen Range | 1585-1634 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 |
| Specificity | POLR2A Antibody detects endogenous levels of total POLR2A protein. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | RPB1 |
| Database Links |
Gene ID: 5430 Swiss-Prot #: P24928 OMIM #: 180660 Unigene #: Hs.270017 |
| Target Modification | Unmodified/Total |
| Modification Sites | |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of HeLa cells, using POLR2A Antibody. The picture on the right is blocked with the synthesized peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using POLR2A Antibody. The picture on the right is blocked with the synthesized peptide. |
| Image 3 |
 Western blot analysis of lysates from COS7 cells, treated with EGF 200ng/ml 30', using POLR2A Antibody. The lane on the right is blocked with the synthesized peptide. |
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