Sulfo-Cyanine5.5isawater-soluble,hydrophilicfluorophorewhichpossessesemissioninfarredareaofthespectrum,ananalogofCy5.5®.Asothercyaninedyes,sulfo-Cyanine5.5hasanoutstandingmolarextinctioncoefficientwhichgivesrisetoitsbrightfluorescence.
Themoleculecontainsfoursulfogroupsthatprovidehydrophilicityandnegativechargetothefluorophore-thisminimizesnon-specificbinding.
Theazidegroupofsulfo-Cyanine5.5azidecanbeconjugatedwithterminalalkynesinthepresenceofcopper(I)catalyst,orwithcycloalkynesincopperfreestrainpromotedreaction.
Generalproperties
| Appearance: | darkcoloredsolid |
| MassspecM+increment: | 984.2 |
| Molecularweight: | 1099.41 |
| Molecularformula: | C43H45K3N6O13S4 |
| Solubility: | goodinwater,DMF,DMSO |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
| Excitationmaximum,nm: | 673 |
| ε,L⋅mol−1⋅cm−1: | 195000 |
| Emissionmaximum,nm: | 691 |
| CF260: | 0.09 |
| CF280: | 0.11 |