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            當前位置 : Millipore >>> Millipore/CBL1512Z | Anti-Integrin αM [CD11b] Antibody, clone OX-42/CBL1512Z/100 µg
            Millipore/CBL1512Z | Anti-Integrin αM [CD11b] Antibody, clone OX-42/CBL1512Z/100 µg
            • Millipore/CBL1512Z | Anti-Integrin αM [CD11b] Antibody, clone OX-42/CBL1512Z/100 µg

            Millipore/CBL1512Z | Anti-Integrin αM [CD11b] Antibody, clone OX-42/CBL1512Z/100 µg

            價格: ¥3324.00 市場價: 5540.00

            貨號: CBL1512Z
            品牌: Millipore
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              • Description
                CatalogueNumberCBL1512Z
                ReplacesMAB1405Z
                BrandFamilyChemicon®
                TradeName
                • Chemicon
                DescriptionAnti-IntegrinαM[CD11b]Antibody,cloneOX-42
                AlternateNames
                • CD11b
                ProductInformation
                FormatPurified
                PresentationTheimmunoglobulinwaspurifiedbyproteinGaffinitychromatographyandispresentedasaliquidinphosphatebufferedsalinecontainingnopreservatives.
                StorageandShippingInformation
                StorageConditionsMaintaininundilutedaliquotsat-20°Cforupto12monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.
                Applications
                ApplicationDetectIntegrinαM[CD11b]usingthisAnti-IntegrinαMCD11bAntibody,cloneOX-42validatedforuseinFC,IH,IH(P)&IP.
                KeyApplications
                • FlowCytometry
                • Immunoprecipitation
                • Immunohistochemistry(Paraffin)
                ApplicationNotesFlowcytometry

                Immunohistochemicalstudiesofratfreshfrozentissuesectionsandparaffin-embeddedtissuesectionsfollowingeitherperiodate-lysine-paraformaldehyde(PLP)fixation,oracetone.WorksonverylightlyPFAfixed,frozentissues.(perfusiononly4%PFA10-15"nopost-fix).

                Immunoprecipitation:userabbitanti-mouseoranti-mouseIgGbeadforcaptureonly.TheuseofproteinAorproteinGisnotrecommended.

                Optimalworkingdilutionsmustbedeterminedbytheenduser.
                BIOLOGicalInformation
                ImmunogenRatperitonealmacrophages
                CloneOX-42
                ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
                HostMouse
                SpecificityThisantibodyrecognizesmostmacrophages(includingresidentperitonealandactivatedmacrophages),Kupffercells,butonlyabout35%ofalveolarmacrophages.Theantibodyalsolabelsdendriticcellsextensively,granulocytesandcellswiththemorphologyofmicrogliainbrain.Theantibodyprecipitatesthreepolypeptidesof150,103and95KDa.TheantibodyinhibitscomplementmediatedrosettesandisprobablytheratequivalentofthehumanreceptorforIC3bcalledCR3.FUSIONPARTNER:NSOmyelomacellline.
                IsotypeIgG2a
                SpeciesReactivity
                • Rat
                AntibodyTypeMonoclonalAntibody
                EntrezGeneNumber
                EntrezGeneSummaryThisgeneencodestheintegrinalphaMchain.Integrinsareheterodimericintegralmembraneproteinscomposedofanalphachainandabetachain.ThisI-domaincontainingalphaintegrincombineswiththebeta2chain(ITGB2)toformaleukocyte-specificintegrinreferredtoasmacrophagereceptor1("Mac-1"),orinactivated-C3b(iC3b)receptor3("CR3").ThealphaMbeta2integrinisimportantintheadherenceofneutrophilsandmonocytestostimulatedendothelium,andalsointhephagocytosisofcomplementcoatedparticles.SomeintegrinalphaMproteinscontainaninsertedaminoacid(Q,betweenAApositions499and500)resultingfroma3bpshiftinaspliceacceptorthatinsertsthecodonCAG;receptorswithorwithouttheextraaminoacidarefunctionallyindistinguishable
                GeneSymbol
                • ITGAM
                • MO1A
                • CD11b
                • MAC1A
                • CD11B
                • MAC-1
                • CR3A
                • MGC117044
                UniProtNumber
                UniProtSummaryFUNCTION:SwissProt:P11215#Integrinalpha-M/beta-2isimplicatedinvariousadhesiveinteractionsofmonocytes,macrophagesandgranulocytesaswellasinmediatingtheuptakeofcomplement-coatedparticles.ItisidenticalwithCR-3,thereceptorfortheiC3bfragmentofthethirdcomplementcomponent.ItprobablyrecognizestheR-G-DpeptideinC3b.Integrinalpha-M/beta-2isalsoareceptorforfibrinogen,factorXandICAM1.ItrecognizesP1andP2peptidesoffibrinogengammachain.
                SIZE:1152aminoacids;127179Da
                SUBUNIT:Heterodimerofanalphaandabetasubunit.Alpha-Massociateswithbeta-2.
                SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
                TISSUESPECIFICITY:Predominantlyexpressedinmonocytesandgranulocytes.
                DOMAIN:SwissProt:P11215TheintegrinI-domain(insert)isaVWFAdomain.IntegrinswithI-domainsdonotundergoproteasecleavage.
                SIMILARITY:Belongstotheintegrinalphachainfamily.&Contains7FG-GAPrepeats.&Contains1VWFAdomain.
                PhysicochemicalInformation
                Dimensions
                MaterialsInformation
                MaterialsInformation
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