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Millipore/CC120 | Caspase 9, recombinant human active/CC120/25 units

價格: ￥3072.00 市場價: 5120.00

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貨號: CC120

品牌: Millipore

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Description
CatalogueNumber	CC120
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Caspase9,recombinanthumanactive
Overview	Caspase-9isamemberoftheinterleukin-1betaconvertingenzyme(ICE)familyofcysteineproteases.Similartoothercaspases,caspase-9alsoexistsincellsasaninactiveproenzyme.Duringtheinitiationofapoptosistheproenzymeisprocessedataspartateresiduestoformactivecaspase-9.Asoneoftheinitiatorcaspases,activecaspase-9functionstotriggertheactivationofdownstreameffectorcaspasesandfurtherleadtodisassemblyofcellstructures. Thepartiallypurifiedactiverecombinanthumancaspase-9withfull-lengthHIS-6tagwasexpressedinE.coli.Theexpressedcaspase-9spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicofthenativeenzyme.Incombinationwithcaspase-9activityassays,theactiverecombinantcaspase-9isusefulinBIOLOGicalscreeningofcaspaseinhibitors.Therecombinantenzymecanalsobeusedasapositivecontrolincaspase-9assays.Caspase9isknowntohavestrongreactivitywithothersequencepeptides,especiallyDEVD-pNA.
AlternateNames	APAF-3 ICE-LAP6 Mch-6

ProductInformation
Presentation	Lyophilized.Reconstitutewith25μlofPBS+15%glycerol.

StorageandShippingInformation
StorageConditions	Maintainat-70°Cinundilutedaliquotsfor3-6monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.

Applications
KeyApplications	ActivityAssay
ApplicationNotes	Enzymeispreparedasanactivitystandardonly,anyotherapplicationnottested.Caspase-9issubjecttoauto-activationthusactivecaspasesubunitsandpartiallyactiveintermediateswillbeseen.Bandsofapproximately45kDa(pro),35kDa(partiallyactive)and18kDa+12kDa(largeandsmallsubunits)maybeseenbywesternblotdependingupontheantibodyused.Thesmallsubunitisextremelydifficulttodetectinmostwesterns. Activation:incubate1-5μLofstockinPBSpH7.5(100mMHEPEScanbeusedaswell)plus10mMATPand5mMDTT(addedjustbeforeuse)for30minutesat37°C.

Applications

KeyApplications

ActivityAssay

ApplicationNotes

Enzymeispreparedasanactivitystandardonly,anyotherapplicationnottested.Caspase-9issubjecttoauto-activationthusactivecaspasesubunitsandpartiallyactiveintermediateswillbeseen.Bandsofapproximately45kDa(pro),35kDa(partiallyactive)and18kDa+12kDa(largeandsmallsubunits)maybeseenbywesternblotdependingupontheantibodyused.Thesmallsubunitisextremelydifficulttodetectinmostwesterns.

Activation:incubate1-5μLofstockinPBSpH7.5(100mMHEPEScanbeusedaswell)plus10mMATPand5mMDTT(addedjustbeforeuse)for30minutesat37°C.

BiologicalInformation
Purity	10-20%caspase-9proteinbygelanalysis.AlsocontainsE.coliproteins.Approximately,0.8-2μgtotalprotein/unitofactivity.
Source	Human
SpecificActivity	Oneunitoftherecombinantcaspase-9istheenzymeactivitythatcleaves1nmolofthecaspasesubstrateLEHD-pNA(pNA:pnitroanaline)perhourat37°Catsaturatedsubstrateconcentrations.
EntrezGeneNumber	NM_001229.2 NM_032996.1
EntrezGeneSummary	Thisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.ThisproteinisprocessedbycaspaseAPAF1;thisstepisthoughttobeoneoftheearliestinthecaspaseactivationcascade.Alternativesplicingresultsintwotranscriptvariantswhichencodedifferentisoforms.
GeneSymbol	CASP9 CASP-9 ICE-LAP6 MCH6 APAF3 CASPASE-9c OTTHUMP00000044594 APAF-3 EC3.4.22.62[Contains:Caspase-9subunitp35 Caspase-9subunitp10].
UniProtNumber	P55211
UniProtSummary	FUNCTION:SwissProt:P55211#Isoform2lacksactivityisandominant-negativeinhibitorofcaspase-9. SIZE:416aminoacids;46281Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya35kDa(p35)anda10kDa(p10)subunit.Caspase-9andAPAF1bindtoeachotherviatheirrespectiveNH2-terminalCED-3homologousdomainsinthepresenceofcytochromeCandATP.InteractswiththeinhibitorsBIRC2,BIRC4,BIRC5andBIRC7. TISSUESPECIFICITY:Ubiquitous,withhighestexpressionintheheart,moderateexpressioninliver,skeletalmuscle,andpancreas.Lowlevelsinallothertissues. PTM:CleavagesatAsp-315bygranzymeBandatAsp-330bycaspase-3generatethetwoactivesubunits.Caspase-8and-10canalsobeinvolvedintheseprocessingevents. SIMILARITY:SwissProt:P55211##BelongstothepeptidaseC14family.&Contains1CARDdomain.

PhysicochemicalInformation

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Millipore/CC120 | Caspase 9, recombinant human active/CC120/25 units

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