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            當前位置 : Millipore >>> Millipore/CC127 | Caspase 2, recombinant human active/CC127/25 units
            Millipore/CC127 | Caspase 2, recombinant human active/CC127/25 units
            • Millipore/CC127 | Caspase 2, recombinant human active/CC127/25 units

            Millipore/CC127 | Caspase 2, recombinant human active/CC127/25 units

            價格: ¥3576.00 市場價: 5960.00

            貨號: CC127
            品牌: Millipore
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              • Description
                CatalogueNumberCC127
                BrandFamilyChemicon®
                TradeName
                • Chemicon
                DescriptionCaspase2,recombinanthumanactive
                OverviewCaspase-2(alsoknownasICH-1,NEDD-2)isamemberoftheinterleukin-1betaconvertingenzyme(ICE)familyofcysteinproteases.Similarasothercaspases,caspase-2alsoexistsincellsasaninactiveproenzyme.Duringapoptosis,procaspase-2isprocessedataspartateresiduesbyself-proteolysisand/orcleavagebyupstreamcaspases.Theprocessedformofcaspase-2consistsoflarge(19kDa)andsmall(12kDa)subunitswhichassociatetoformtheactiveenzyme.Theactiverecombinanthumancaspase-2wasexpressedinE.coli.Theexpressedcaspase-2spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicofthenativeenzyme.Therecombinantcaspase-2preferentiallycleavesthesubstratesconsistingofconsensussequenceVDVAD(e.g.,VDVAD-AFCandVDVAD-pNA).Therecombinantenzymecanalsobeusedasapositivecontrolincaspaseassaysorindeterminingthespecificityofcaspasesubstrates.Recommendedusage:0.5-1unitforfluorometriccaspaseassaysand2-4unitsforcolorimetriccaspaseassays.Optimalworkingdilutionsmustbedeterminedbyenduser.
                ProductInformation
                PresentationPartiallypurifiedrecombinantcaspase2withafulllengthHIS-6-tag.Approximately10-20%caspase2protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity.Lyophilized.Reconstitutein25μLPBScontaining15%glycerol.
                StorageandShippingInformation
                StorageConditionsMaintainunopenedandrehydratedproductat-70°Cinundiluted,freeze-thawprotected,tightlysealedaliquots.Avoidrepeatedfreeze/thawcycles.Productstablefor6monthsfromdateofpurchasedifhandledappropriately.
                Applications
                KeyApplications
                • ActivityAssay
                BIOLOGicalInformation
                PurityPartiallypurifiedrecombinantcaspase3withafulllengthHIS-6-tag.Approximately10-20%caspase2protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity.
                SpecificActivity25U:Whereoneunitoftherecombinantcaspase-2istheenzymeactivitythatcleaves1nmolofthecaspasesubstrateVDVAD-pNA(pNA:pnitroanaline)perhourat37oCinareactionsolutioncontaining50mMHepes,pH7.2,50mMNaCl,0.1%Chaps,10mMEDTA,5%Glycerol,and10mMDTT.
                EntrezGeneNumber
                EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Theproteolyticcleavageofthisproteinisinducedbyavarietyofapoptoticstimuli.Alternativesplicingofthisgeneresultsinmultipletranscriptvariantsthatencodedifferentisoforms.
                GeneSymbol
                • CASP2
                • ICH1
                • NEDD2
                • NEDD-2
                • ICH-1L/1S
                • CASP-2
                • ICH-1L
                • EC3.4.22.55[Contains:Caspase-2subunitp18
                • Caspase-2subunitp13
                • Caspase-2subunitp12].
                UniProtNumber
                UniProtSummaryFUNCTION:SwissProt:P42575#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Mightfunctionbyeitheractivatingsomeproteinsrequiredforcelldeathorinactivatingproteinsnecessaryforcellsurvival.
                SIZE:452aminoacids;50685Da
                SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbyap18subunitandap12subunit.InteractswithLRDD.
                TISSUESPECIFICITY:Expressedinlargeramountsintheembryoniclung,liverandkidneythanintheheartandbrain.Intheadultshigherlevelexpressionisseenintheplacenta,lung,kidney,pancreasthanintheheart,brain,liverandskeletalmuscle.
                PTM:Thematureproteasecanprocessitsownpropeptide,butnotthatofothercaspases.
                SIMILARITY:SwissProt:P42575##BelongstothepeptidaseC14family.&Contains1CARDdomain.
                PhysicochemicalInformation
                Dimensions
                MaterialsInformation
                MaterialsInformation
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