當前位置： Millipore >>> Millipore/CYT420 | ChemiKine? Pigment Epithelium Derived Factor, Sandwich ELISA/CYT420/1 kit

Millipore/CYT420 | <i>ChemiKine?</i> Pigment Epithelium Derived Factor, Sandwich ELISA/CYT420/1 kit

Millipore/CYT420 | ChemiKine? Pigment Epithelium Derived Factor, Sandwich ELISA/CYT420/1 kit

價格: ￥5244.00 市場價: 8740.00

打印產品信息

貨號: CYT420

品牌: Millipore

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即購買加入購物車

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

Description
CatalogueNumber	CYT420
BrandFamily	Chemicon®
TradeName	ChemiKine Chemicon
Description	ChemiKine?PigmentEpitheliumDerivedFactor,SandwichELISA
Overview	Introduction PigmentEpithelium-DerivedFactor(PEDF)wasfirstidentifiedasafactorsecretedbyprimaryretinalpigmentepithelial(RPE)cellsthatpromotesthesurvivalofneuronsinvitroandinvivo.MolecularcharacterizationofPEDFshowsittobea50kDamemberoftheserpinfamilyofproteaseinhibitors1.AsecondmodeofactionofPEDFwasidentifiedwiththedemonstrationthatPEDFinducesapoptosisofmicrovascularendothelialcells,apropertythatmaintainstheavascularnatureoftheretina2.Asanumberofophthalmologicdisordersarecharacterizedbydysregulationoftheretinalinnervationandmicrovasculature,PEDFservesasanimportantregulatorofhumaneyedisease3.Inaddition,PEDFappearstohavemultifunctionalantitumoractivityinaneuroblastomamodel,asSchwanncell-derivedPEDFinducesadifferentiated,lessmalignantphenotypeinneuroblastomacells,promotesfurthergrowthandsurvivalofSchwanncells,andinhibitsangiogenesis4. ChemiKine?PigmentEpithelium-DerivedFactor(PEDF)kitisasandwichstripwell-typeenzymeimmunoassay(EIA),whichmeasuresPEDF.ThekitwillmeasurePEDFofvitreousfluidofhumanorigin. TestPrinciple WiththeChemiKine?PEDFassaysystem,amousemonoclonalantibodygeneratedagainsthumanPEDFiscoatedontoamicroplateandisusedtocapturePEDFfromasample.Asecond,biotinylatedPEDF-specificmousemonoclonalantibodydetectsthecapturedPEDF.Peroxidase-labeledstreptavidindetectsthebiotinylatedmousemonoclonalantibody.AfteradditionofthesubstrateandstopsolutiontheamountofPEDFisdetermined.ThestandardcurvedemonstratesadirectrelationshipbetweenOpticalDensity(OD)andPEDFconcentration:i.e.,thehighertheODthehigherthePEDFconcentrationinthesample.
AlternateNames	PEDF
MaterialsRequiredbutNotDelivered	1.Multi-channelorrepeatingPipettes 2.37oCincubator 3.Platesealers 4.Pipettors&tipscapableofaccuratelymeasuring10-1000mL 5.GraduatedSEROlogicalpipettes 6.96-wellmicroplatereaderwith450nmfilter 7.Graphpaperformanualplottingofdata 8.Polypropylenetesttubesforstandardandsampledilutions 9.Mechanicalvortex 10.One1or2litercontainer 11.Urea

ProductInformation
Components	PEDFELISAPlate:(PartNo.61597)One96-WellImmunoplatePre-coatedwithMouseanti-HumanPEDFMonoclonalAntibody,sealedinafoilpouch. WashBufferConcentrate:(PartNo.60245)One100mL(10X)bottleofConcentrate. AssayDiluent:(PartNo.60240)One60mLbottle(ReadytoUse). PEDFStandard(Human):(PartNo.61600)Onevialcontaining250ngrecombinantfulllengthhumanPEDF(Lyophilized). BiotinylatedMouseanti-HumanPEDFMonoclonalAntibody:(PartNo.61598)One50μLvial. Streptavidin,HRPconjugated:(PartNo.61599)One50μLvial. TMB/ESolution:(PartNo.60096)One10mLbottleofaReadytoUsesolutionof3,3",5,5"-tetramethylbenzidineinaproprietarybufferwithenhancer. StopSolution(PartNo.60193):One12mLbottleofanHClsolution(ReadytoUse).
Detectionmethod	Colorimetric

ProductInformation

Components

PEDFELISAPlate:(PartNo.61597)One96-WellImmunoplatePre-coatedwithMouseanti-HumanPEDFMonoclonalAntibody,sealedinafoilpouch.
WashBufferConcentrate:(PartNo.60245)One100mL(10X)bottleofConcentrate.
AssayDiluent:(PartNo.60240)One60mLbottle(ReadytoUse).
PEDFStandard(Human):(PartNo.61600)Onevialcontaining250ngrecombinantfulllengthhumanPEDF(Lyophilized).
BiotinylatedMouseanti-HumanPEDFMonoclonalAntibody:(PartNo.61598)One50μLvial.
Streptavidin,HRPconjugated:(PartNo.61599)One50μLvial.
TMB/ESolution:(PartNo.60096)One10mLbottleofaReadytoUsesolutionof3,3",5,5"-tetramethylbenzidineinaproprietarybufferwithenhancer.
StopSolution(PartNo.60193):One12mLbottleofanHClsolution(ReadytoUse).

Detectionmethod

Colorimetric

StorageandShippingInformation
StorageConditions	Maintaintheunopenedkitat2-8°Cuntilexpirationdateindicatedonthelabel.Afteropeningthekitmaintaineachcomponentat2-8°Cuntilexpirationdateindicatedonthelabel.Afterreconstitution,maintainthePEDFStandardat-20oCforupto30days.Thestandardmaybefrozenandthawedthreetimeswithoutlossofimmunoreactivity.Donotstorereconstitutedstandardat4oC,aslossofimmunoreactivitywillresult. Precautions ·WashBufferandAssayDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly. ·Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata.

StorageandShippingInformation

StorageConditions

Maintaintheunopenedkitat2-8°Cuntilexpirationdateindicatedonthelabel.Afteropeningthekitmaintaineachcomponentat2-8°Cuntilexpirationdateindicatedonthelabel.Afterreconstitution,maintainthePEDFStandardat-20oCforupto30days.Thestandardmaybefrozenandthawedthreetimeswithoutlossofimmunoreactivity.Donotstorereconstitutedstandardat4oC,aslossofimmunoreactivitywillresult.

Precautions

·WashBufferandAssayDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly.

·Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata.

Applications
Application	ThisChemiKinePigmentEpitheliumDerivedFactor,SandwichELISAisusedtomeasure&quantifyPEDFlevelsinNeuroscienceresearch.
KeyApplications	ELISA
ApplicationNotes	ChemiKine?PEDFkitisdesignedtomeasuretheamountofPEDFincellculturesupernatants,tissuehomogenatesandBIOLOGicalfluid(vitreous,serum,plasma,andserum-free)samplesofhumanorigin.Thereareenoughreagentsincludedinthiskitforone96-wellimmunoassayplate.Runningduplicatewellsforsamplesandstandardsisrecommended. ForResearchUseOnly.Notforuseindiagnosticprocedures.

BiologicalInformation
Host	Mouse
SpeciesReactivity	Human
AntibodyType	MonoclonalAntibody
AnalytesAvailable	PEDF
EntrezGeneNumber	NM_002615.4
GeneSymbol	SERPINF1 Serpin-F1 PEDF PIG35 EPC-1
UniProtNumber	P36955
UniProtSummary	FUNCTION:SwissProt:P36955#Neurotrophicprotein;inducesextensiveneuronaldifferentiationinretinoblastomacells.Potentinhibitorofangiogenesis.AsitdoesnotundergotheS(stressed)toR(relaxed)conformationaltransitioncharacteristicofactiveserpins,itexhibitsnoserineproteaseinhibitoryactivity. SIZE:418aminoacids;46342Da SUBCELLULARLOCATION:Secreted.Melanosome.Note=EnrichedinstageImelanosomes. TISSUESPECIFICITY:Retinalpigmentepithelialcellsandbloodplasma. DEVELOPMENTALSTAGE:Expressedinquiescentcells. DOMAIN:SwissProt:P36955TheN-terminal(AA44-121)exhibitsneuriteoutgrowth-inducingactivity.TheC-terminalexposedloop(AA382-418)isessentialforserpinactivity. PTM:TheN-terminusisblocked. SIMILARITY:Belongstotheserpinfamily.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Parafilm M封口膜/PM996/4x125/盒
￥0.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥850.00
ThermoFisherQ32856Qubit? Assay Tubes現貨
￥590.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00

Millipore/CYT420 | ChemiKine? Pigment Epithelium Derived Factor, Sandwich ELISA/CYT420/1 kit

客戶服務

何為188

關注188微信公眾號