The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys23.
Immunogen Range
16-65
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
Histone H3 (Acetyl-Lys23) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at Lys23.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys23) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys23) Antibody. The lane on the right is blocked with the synthesized peptide.