Water-solubleCyanine5dyeazideforClickchemistry.Brightandphotostabledyewithredfluorescence.Thereagenthashighwatersolubility.Duetohighsolubilityandhydrophilicity,thedyecanbeusedforlabelingbiomoleculesinnative,purelyaqueousconditions.
Thisreagent,asopposedtocompetingproducts,issuppliedasanalkalinemetalsaltandnottriethylammoniumsalt.Therefore,itisapowderwhichiseasytohandleanddispense.
Sulfo-Cyanine5isananalogofCy5®,averypopularfluorophore,thereforethisreagentiscompatIBLetoawiderangeofstandardfluorescentinstrumentationsuchasimagers,platereaders,andmicroscopes.
Generalproperties
| Appearance: | darkbluecrystals |
| Molecularweight: | 746.87 |
| Molecularformula: | C35H43N6NaO7S2 |
| Solubility: | veryhighinwater |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight. |
Spectralproperties
| Excitationmaximum,nm: | 646 |
| ε,L⋅mol−1⋅cm−1: | 271000 |
| Emissionmaximum,nm: | 662 |
| Fluorescencequantumyield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |