Peryleneisabrightandextremelyphotostablefluorescentpolycyclicaromatichydrocarbon(PAH)labelwithquantumyieldapproachingquantitative.
Duetolowlifetimeoffluorescence,thisprobedoesnotformexcimers.
Perylenelabelingofbiomoleculesisusedtobedifficultandtediousbecauseoflowsolubilityofperylene,andlowavailABIlityofactivatedderivatives.However,labelingviaClickchemistrywiththisperyleneazideisnearlyaseasy,aswithotherdyes.Labelingpeptide,DNA,proteinwiththispolyaromatichydrocarbonhasneverbeensosimpleyet.
Toincreasehydrophilicity,andfacilitatelabeling,thisPAHazidecontainshydrophilictriethyleneglycollinker.
Generalproperties
| Appearance: | orange-yellowsolid |
| Molecularweight: | 452.50 |
| Molecularformula: | C27H24N4O3 |
| IUPACname: | 3-(8-Azido-3,6-trioxaoctylaminocarbonyl)perylene |
| Solubility: | goodindichloromethaneandchloroform,moderateinDMSO,DMF,andacetonitrile |
| Qualitycontrol: | NMR1H(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight. |
Spectralproperties
| Excitationmaximum,nm: | 435;408;252 |
| Emissionmaximum,nm: | 439;467 |
| Fluorescencequantumyield: | 1.0 |