Synonym:
o-Dianisidine bis(diazotized) zinc double salt,
Azoic Diazo No. 48,
DBB,
Diazo Blue B,
Naphthanil Diazo Blue B
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CAS Number 14263-94-6
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Linear Formula C14H12N4O2Cl2 · ZnCl2
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Molecular Weight 475.47
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Beilstein Registry Number 1499067
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Colour Index Number 37235
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EC Number 238-153-2
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MDL number MFCD00075203
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PubChem Substance ID 24894113
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Popular Documents: Specification Sheet (PDF)
Properties:
Related Categories |
Aflatoxins,
Analytical Reagents,
Analytical/Chromatography,
Cell Biology,
Derivatization Reagents,
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description
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Technical grade
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InChI Key
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GPPKNJIWDULNQH-UHFFFAOYSA-J
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form
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powder
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composition
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Dye content, ~95%
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mp
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>300 °C(lit.)
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solubility
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H2O: 1 mg/mL
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storage temp.
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2-8°C
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Description:
Packaging
10, 100 g in glass bottle
Application
• Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
• It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
• It has also been used for the determination of the lipase activity.
Biochem/physiol Actions
Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.
Safety:
Symbol

GHS08
Precautionary statements
P201-P280-P308 + P313
Personal Protective Equipment
Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
RIDADR
NONH for all modes of transport