Withitsquantumyieldapproachingunity,ROXhasbecomeapopulardyeforqPCRandmicroscopy.Thisbrightfluorophoreisavailableasanalkyneforcopper-catalyzedClickchemistryreaction.
Thisisapure5-isomerofROX.
ROXispronetooxidation.Therefore,itsderivativesshouldbepreferablystoredininertatmosphere.
Generalproperties
| Appearance: | purplesolid |
| MassspecM+increment: | 571.3 |
| Molecularweight: | 571.66 |
| Molecularformula: | C36H33N3O4 |
| Solubility: | goodinDMSO,DMF,methanol,ethanol |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
| Excitationmaximum,nm: | 570 |
| ε,L⋅mol−1⋅cm−1: | 80000 |
| Emissionmaximum,nm: | 591 |
| Fluorescencequantumyield: | 1.00 |