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NEB/NEBNext? Ultra? Directional?RNA Library Prep Kit for Illumina?/E7420L/96 reactions

價格: ￥44940.00 市場價: 74900.00

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貨號: E7420L-96reactions

品牌: NEB

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Description:

TheNEBNext?Ultra??Directional?RNALibraryPrepKitforIllumina?containsenzymesandbuffersthatareideallysuitedforCDNAlibrarypreparationfornext-generationsequencing.Eachofthesecomponentsmustpassrigorousqualitycontrolstandardsandarelotcontrolled,bothindividuallyandasasetofreagents.

NEBNext Ultra? Directional RNA produces libraries with high directionality/strand specificity — UltraDirectionalRNAlibrariesofmRNApurifiedfromUniversalHumanReferenceRNA(**Agilent**??#740000),weresequencedontheIlluminaplatform.Thehighpercentageofreadsmappedtothe?expectedstrandshowsthehighstrand-specificityofthelibraries.ActinomycinDisrequiredto?achievemaximalstrandspecificity.

NEBNext Ultra? Directional RNA libraries provide even coverage across transcripts — mRNAwasisolatedfromUniversalHumanReferenceRNA(Agilent#740000)andlibrariesweremade?usingtheNEBNextUltra?DirectionalRNAKitandtheIlluminaTruSeqStrandedmRNAKit.Libraries?weresequenced(singleendreads)ontheIlluminaplatform(GAIIx).Goodcoverageacrossexpressed?transcriptswasobtained.

Low input NEBNext Ultra Directional RNA libraries retain complexity — RibosomalRNA-depletedRNAlibrariesweremade?from10ngor100ngUniversalHumanReferenceRNA?(Agilent#740000)usingtheNEBNextUltraDirectional?RNAKit.TranscriptabundancesinFragmentsPer?KilobaseofexonperMillionfragmentsmapped?(FPKM)showstrongconcordance(R²=0.90745),?indicatingretentionoflibrarycomplexityevenatlow?inputamount.

Equivalent transcript representation is achieved between technical replicates of NEBNext Ultra Directional RNA libraries — TechnicalreplicatesofmRNAlibrariesmadefrom?mRNAisolatedfromUniversalHumanReference?RNA(Agilent#740000)showstrongconcordance?(R²=0.99409).

Sequencing data shows strong concordance between NEBNext Ultra Directional RNA and Illumina TruSeq Stranded RNA libraries — mRNAwasisolatedfromUniversalHumanReference?RNA(Agilent#740000)andlibrariesweremadeusing?theNEBNextUltraDirectionalRNAKitandtheIllumina?TruSeqStrandedmRNAKit.ThisFPKMconcordance?plotshowscomparabletranscriptrepresentation?(R²=0.96009)betweenthelibraries.

FunctionalValidation
EachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofanRNAlibraryontheIlluminasequencingplatform.

LotControl

Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage

ReagentsSupplied

Thefollowingreagentsaresuppliedwiththisproduct:

	Storeat(°C)	Concentration
NEBNextFirstStrandSynthesisReactionBuffer	-20
RandomPrimers	-20
ProtoScript?IIReverseTranscriptase	-20	200,000units/ml
MurineRNaseInhibitor	-20
NEBNextSecondStrandSynthesisEnzymeMix	-20
NEBNextSecondStrandSynthesisReactionBufferwithdUTPMix	-20	10X
NEBNextEndPrepEnzymeMix	-20
NEBNextEndRepairReactionBuffer	-20	10X
Blunt/TALigaseMasterMix	-20
Nuclease-freeWater	-20
NEBNextUSER^?Enzyme	-20
NEBNext®Q5®HotStartHiFiPCRMasterMix	-20

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NEB/NEBNext? Ultra? Directional?RNA Library Prep Kit for Illumina?/E7420L/96 reactions

Description:

LotControl

ReagentsSupplied

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