當前位置： NEB >>> NEB/NEBNext??Library Quant Kit for Illumina?/E7630S/100 reactions

NEB/NEBNext??Library Quant Kit for Illumina?/E7630S/100 reactions

價格: ￥1188.00 市場價: 1980.00

打印產品信息

貨號: E7630S-100reactions

品牌: NEB

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即購買加入購物車

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

Description:

TypicalresultsfromtheNEBNextLibraryQuantKitonaBio-RadCFX96Touch(A)andanAppliedBiosystems7500Fastreal-timeqPCRinstrument(B).Amplificationcurvesareshownontheleftandresultingstandardcurvesontheright.Alldefaultsettingswereusedonbothplatforms.ABI7500Fastassayincluded1XROX(LowConcentration)andtheROXnormalization.

Accuratequantitationofnext-generationsequencinglibrariesisessentialformaximizingdataoutputandqualityfromeachsequencingrun.ForIlluminasequencingspecifically,accuratequantitationoflibrariesiscriticaltoachieveoptimalclusterdensities,arequirementforoptimalsequenceperformance.qPCRisconsideredtobethemostaccurateandeffectivemethodoflibraryquantitation,providingconsiderablyhigherconsistencyandreproducibilitythanelectrophoresisorspectrophotometry,whichmeasuretotalnucleicacidconcentration.Amplification-basedmethodsquantitateonlythosemoleculesthatcontainbothadaptorsequences,therebyprovidingamoreaccurateestimateoftheconcentrationofthelibrarymoleculesthatcanbesequenced.

TheNEBNextLibraryQuantKitdeliverssignificantimprovementstoqPCR-basedlibraryquantitationfornextgensequencing.TheNEBNextLibraryQuantKitforIlluminacontainscomponentsthatareoptimizedforqPCR-basedquantitationoflibrariespreparedforIlluminanext-generationsequencingplatforms.TheNEBNextLibraryQuantKitcontainsprimerswhichtargettheP5andP7Illuminaadaptorsequencesandasetofhigh-quality,pre-dilutedDNAstandardstoenablereliablequantitationofdilutedDNAlibrariesbetween150–1000bp.?

Features

ProvidesmoreaccurateandreproducIBLequantvaluesthanalternativemethodsandkits
CompatiblewithlibrarieswithabroadrangeofinsertsizesandGCcontent,madebyavarietyofmethods
Requiresonly4standards,allowingmorelibrariestobequantitatedperkit?
Suppliedwithaconvenient,LibraryDilutionBuffer
TheNEBNextLibraryQuantMasterMixrequiresonlytheadditionofprimers
Utilizesasingleextensiontimeforalllibraries,regardlessofinsertsize
LibraryquantvaluescanbeeasilycalculatedusingNEB’sonlinetool,atNEBioCalculator.neb.com
ROXisincludedinthekit,forusewithqPCRinstrumentsthatrequireareferencedyefornormalization

NEBNext Library Quant Kit workflow — **NEBNextLibraryQuantKitworkflow?**

qPCR provides more consistent library quantitation results than Bioanalyzer? analysis — Concentrationsof4librariesweredeterminedbytheNEBNextLibraryQuantKit(orange)andcomparedtovaluesmeasuredusingtheAglientBioanalyzer(blue).ComparedtoNEBNext’sqPCR-basedmethod,theBioanalyzerconcentrationsdisplayedagreaterlevelofvariation.

Greater reproducibility of library quantitation with the NEBNext Library Quant Kit — Three340–400bplibrarieswerequantitatedby4differentusers2–4timesusingeithertheNEBNextorKapaTMLibraryQuantificationKit(Universal).AnotableimprovementinquantitationconsistencywasobservedforconcentrationsdeterminedbytheNEBNextKit(orange)versusthosefromtheKapakit(gray).?

Greater lot-to-lot consistency of standards with the NEBNext Library Quant Kit — AccurateqPCRquantitationrequirestheuseofhigh-qualityDNAstandardswithknownconcentrations.TheNEBNextLibraryQuantKitcontains4standardsproducedwithahighlevelofbothquantitationaccuracyandconsistency.Thisfigureshowsdatafrom>70totalrunsfrom4lotsofbothNEBNext(orange)andKapa(gray)standards,withallC_qvaluesplotted.Boxandwhiskersindicatemeanandquartiles.TheNEBNextLibraryStandardsdisplayedmuchlowervariationinC_q,resultinginmoreconsistentquantitationperformance.?

The NEBNext Library Quant Kit values enable optimal cluster densities — SevendifferentlibrarieswerequantitatedusingeithertheNEBNextLibraryQuantKit(orange)ortheKapaLibraryQuantificationKit(Universal)(gray).Undilutedlibraryconcentrationsrangedfrom2–200nM.Librariesweredilutedto8pMandloadedontoaMiSeq?instrument(v2chemistry;MCSv2.4.1.3).LibrariesquantitatedwiththeNEBNextkitresultedinarawclusterdensityaverageof1160k/mm2,directlyintheoptimalrangeof900–1300k/mm2.Incontrast,librariesloadedbasedontheKapaquantitationaveragedonly660k/mm2.?

Accurate Library Quantitation is achieved with a broad range of library size and GC content — AselectionoflibrariessuccessfullyquantitatedwiththeNEBNextLibraryQuantKit.Librariesareplottedbysize,rangingfromsmallerlibraries(sRNA,FFPE)at150–230bptolargestlibrariesat980bp.Variousinputsourceswereused,rangingfrom20-70%GCcontent,asindicatedbytextcolor.LibrarieswerepreparedusingNEBNext,IlluminaTruSeq?NanoandKapaHyperlibraryprepkits(datanotshown).NodependenceonsizeorGCcontentwasobservedinlibraryquantitationwhenusingNEBNext.

LotControl
ThelotsprovidedintheNEBNextLibraryQuantKitforIlluminaaremanagedseparatelyandarequalifiedbyfunctionaltesting.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaasdescribedoneachindividualcomponentpageinthemanual.

FunctionallyValidated
EachsetofreagentsisfunctionallyvalidatedtogetherthroughqPCR-basedlibraryquantitationassays.Reagentspassthefunctionaltestbyadheringtostringentcriteriasetforassayefficiencyandquantitationcycles(C_q)foreachDNAstandardandnotemplatecontrolreaction.

Forlargervolumerequirements,customizedandbulkpackagingisavailablebypurchasingthroughtheOEM/BulksdepartmentatNEB.Pleasecontactnebsolutions@neb.comforfurtherinformation.

ReagentsSupplied

Thefollowingreagentsaresuppliedwiththisproduct:

	Storeat(°C)	Concentration
NEBNextLibraryQuantMasterMix	-20
NEBNextLibraryQuantPrimerMix	-20
NEBNextLibraryQuantDNAStandard1	-20
NEBNextLibraryQuantDNAStandard2	-20
NEBNextLibraryQuantDNAStandard3	-20
NEBNextLibraryQuantDNAStandard4	-20
ROX(Low)	-20
ROX(High)	-20
NEBNext?LibraryDilutionBuffer	-20	10X

Notes:

StorageofKitComponents:AlloftheindividualcomponentsoftheNEBNextLibraryQuantKitforIlluminaarestableat–20°Cfortwoyears.Whennotinuse,kitcomponentsshouldbestoredat–20°C.Allcomponentsofthekit,includingthecombinedNEBNextLibraryQuantMasterMixandPrimerMix,arestableforatleast30freeze/thaws.OncethePrimerMixhasbeenaddedtotheNEBNextLibraryQuantMasterMix(seeProtocolonpage6ofmanual),theresultingmixcanbestoredat–20°Cforsevenmonths.Forshort-termstorage,thismixmaybestoredat4°Cforuptosixweeks.Reactionscanbepreparedatroomtemperature,butunusedportionsofthekitreagentsshouldbekeptoniceafterthawingthemforuse.ForReagentsSupplied:Ifyouneeddilutionbufferinadditiontowhatisprovidedinthekit,itcanbepurchasedseparately.

售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Parafilm M封口膜/PM996/4x125/盒
￥0.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥850.00
ThermoFisherQ32856Qubit? Assay Tubes現貨
￥590.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00

NEB/NEBNext??Library Quant Kit for Illumina?/E7630S/100 reactions

Description:

ReagentsSupplied

Notes:

客戶服務

何為188

關注188微信公眾號