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Millipore/ECM200 | QCM 3 μm Endothelial Cell Migration Assay Fibronectin, Colorimetric/ECM200/1 kitSufficient for 12 ass

價格: ￥5724.00 市場價: 9540.00

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貨號: ECM200

品牌: Millipore

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Description
CatalogueNumber	ECM200
TradeName	QCM Chemicon
Description	QCM3μmEndothelialCellMigrationAssayFibronectin,Colorimetric
Overview	Alsoavailable:CellComb?ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere Introduction Angiogenesisisafundamentalprocessinvolvingthegrowthofnewbloodvesselsfrompre-existingvessels.Itisimportantindevelopmentandwoundhealing,aswellaspathologicdiseasessuchasdiabeticretinopathyandcancer.Duringangiogenesis,endothelialcellsneedtomoveoutofexistingvessels,migrateintonewareas,proliferateandassembleintonewcapillaries.Themigrationofendothelialcellsisregulatedbymanyangiogenicfactorsandanti-angiogenicfactors.Itiscriticalforresearcherstounderstandthemechanismsofendothelialcellmigration. Millipore"s3μmQCM?EndothelialCellMigrationAssay–Fibronectin,ColorimetricprovidesaquickandefficientsystemtostudytheABIlityofcompoundstoinduceorinhibitendothelialcellmigration.Thisassayalsoallowsscreeningofpharmacologicalagents,evaluationofintegrinsorotheradhesionreceptorsresponsIBLeforendothelialcellmigration,analysisofgenefunctionintransfectedcells,anddeterminationofECMproteininvolvementincellmovement. Thisversatileassaypermitscountingofindividualmigratorycells,and,moreimportantly,allowsquantitativeanalysisbyopticaldensity(OD)usingastandardmicroplatereader.Thisconvenientassayallowslargescalescreeningandquantitativecomparisonofmultiplesamplesandincludesindividualmigrationcontrolsforeachsample.
MaterialsRequiredbutNotDelivered	1.PrecisionPipettes:sufficientforaliquotingcells. 2.Harvestingbuffer:EDTAortrypsinbasedcelldetachmentbuffer,orothercelldetachmentformulationsasoptimizedbyindividualinvestigators.Millipore’sready-to-usenon-mammaliandetachmentsolution,Accutase?(Cat.No.SCR005)canalsobeused. 3.Endothelialcells(forexample:HUVECcells). 4.Endotheliumcellculturemediumappropriateforsubjectcells,suchasEGM-2(Endothelialcellgrowthmedia-2) 5.QuenchingMedium:serum-freemedium,suchasDMEM,MEMetccontaining5%BSA.Mustcontaindivalentcations(Mg2+,Ca2+)sufficientforquenchingEDTAinharvestingbuffer. 6.SterilePBSorHBSStowashcells. 7.Distilledwater 8.(Optional)Chemoattractantorpharmacologicalagentaddedtoculturemedium. 9.Lowspeedcentrifugeandtubesforcellharvesting. 10.CO2incubatorappropriateforsubjectcells. 11.Hemocytometerorothermeansofcountingcells. 12.Trypanblueorequivalentviabilitystain. 13.Microplatereader(540-570nmdetection)orspectrophotometer. 14.Sterilecellculturehood 15.(Optional)Graduatedocular(calibrated),orautomatedmethodforcountingstainedcellsonamembrane. 16.Shaker

ProductInformation
Components	1.FibronectinTestPlate:One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples. 2.BSAControlPlate:One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls. 3.CellStainSolution:Onevial-10mL 4.ExtractionBuffer:Onevial-10mL 5.24-WellStainExtractionPlate 6.96-WellStainQuantitationPlate 7.Swabs:50ea 8.Forceps:1pair
Detectionmethod	FluorescentColorimetric

ProductInformation

Components

1.FibronectinTestPlate:One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples.
2.BSAControlPlate:One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls.
3.CellStainSolution:Onevial-10mL
4.ExtractionBuffer:Onevial-10mL
5.24-WellStainExtractionPlate
6.96-WellStainQuantitationPlate
7.Swabs:50ea
8.Forceps:1pair

Detectionmethod

FluorescentColorimetric

StorageandShippingInformation
StorageConditions	Storekitmaterialsat2°to8°Cforuptotheirexpirationdate.Donotfreeze.

Applications
Application	ThisQCM3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.
ApplicationNotes	TheMilliporeQCM?3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The3μmporesizeinthisassayisoptimalforendothelialcellssuchasHUVEC,butnotsufficientforfibroblastmigration.TheMilliporeQCM?3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricassayisintendedforresearchuseonly;notfordiagnosticapplications. Eachkitprovidessufficientmaterialsfortheevaluationof12samples.

Applications

Application

ThisQCM3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.

ApplicationNotes

TheMilliporeQCM?3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The3μmporesizeinthisassayisoptimalforendothelialcellssuchasHUVEC,butnotsufficientforfibroblastmigration.TheMilliporeQCM?3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricassayisintendedforresearchuseonly;notfordiagnosticapplications.
Eachkitprovidessufficientmaterialsfortheevaluationof12samples.

BIOLOGicalInformation

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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Millipore/ECM200 | QCM 3 μm Endothelial Cell Migration Assay Fibronectin, Colorimetric/ECM200/1 kitSufficient for 12 ass

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