當前位置： Millipore >>> Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit

Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit

價格: ￥7560.00 市場價: 12600.00

打印產品信息

貨號: ECM340

品牌: Millipore

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即購買加入購物車

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

Description
CatalogueNumber	ECM340
BrandFamily	Chemicon®
TradeName	Chemicon
Description	HumansVCAM-1ELISA
Overview	Vascularcelladhesionmolecule-1(VCAM-1,CD106)isamemberoftheimmunoglobulingenesuperfamily.TwoformsofVCAM-1withsixorsevenextracellularIg-likedomainsariseduetoalternativesplicingfromaseven-domainVCAM-1(7DVCAM-1).7DVCAM-1isthedominantformexpressedbyculturedhumanendothelialcells[Cybulskyetal.,1991;Hessionetal.,1991].Homologyofdomains1through3withdomains4through6suggestsageneduplicationevent[Cybulskyetal.,1991;Hessionetal.,1991].TheCDNAof7DVCAM-1predictsacoreproteinofapproximately81kDawithsevensitesforN-linkedglycosylation.Uponcompleteglycosylationthematureproteinhasamolecularweightofapproximately102kDa.Thisobservationisingeneralagreementwiththe110kDaproteinimmunoprecipitatedfromcytokine-activatedendothelium[Carlosetal.,1990;Riceetal.,1990]. VCAM-1appearstohavebeenhighlyconservedthroughevolution.BothratandmouseVCAM-1arehighlyhomologousattheproteinleveltothehumanVCAM-1(77%and76%,respectively)[Hessionetal.,1992]. VCAM-1supportstheadhesionoflymphocytes,monocytes,naturalkillercells,eosinophilsandbasophils,throughitsinteractionwithintegrina4b1(VLA-4).VCAM-1/VLA-4interactionmediatesfirmadherenceofcirculatingnon-neutrophilicleukocytestoendothelium[Shararetal.,1995].VCAM-1alsoparticipatesinleukocyteadhesionoutsideofthevasculature,mediatingprecursorlymphocyteadhesiontobonemarrowstromalcells[Ryanetal.1991]andBcellbindingtolymphnodefolliculardendriticcells[Feedmanetal.,1992].VCAM-1isnotconstitutivelyexpressedonendothelium,butcanbeup-regulatedinvitroinresponsetoLPS,TNF-a,andIL-1[Carlos&Harlan,1990;Osbornetal.,1989],aswellastointerferon-gandIL-4[Masinovskyetal.1990].VCAM-1isalsopresentontissuemacrophages,dendritecells,bonemarrowfibroblasts,myoblastsandmyotubes. AsolubleformofVCAM-1hasbeendescribed[DeMaeyer&DeMaeyer-Buignard,1995;Lobbetal.,1991],andsolubleVCAM-1hasbeenfoundinserum[Gearingetal.,1995].IncreasedlevelsofsolubleVCAM-1canbedetectedinseveraldiseasestates,includingcancer[Banksetal.,1993;Fortisetal.,1995;Gearing&Newman,1993;Laietal.,1995],autoimmunedisorders[Gearing&Newman,1993;Doreduffyetal.,1995;Gruschwitzetal.,1995;Masonetal.,1993;Mrowka&Sieberth,1995;Spronketal.,1994;Wellicomeetal.,1993],infections[Jakobsenetal.,1994]andinflammations[Gearing&Newman,1993;Mrowka&Sieberth,1995;Adamsetal.,1995;Limetal.,1995]. TestPrinciple: Ananti-VCAM-1monoclonalantibodyisadsorbedontomicrowells. SolubleVCAM-1presentinasampleorstandardthenbindstoantibodiesadsorbedtothemicrowells.Amixtureofbiotin-conjugatedmonoclonalanti-VCAM-1antibodyandStreptavidin-HRPconjugateisadded.Biotinylatedanti-VCAM-1antibodybindstoVCAM-1capturedbythefirstantibody. Streptavidin-HRPbindstothebiotinylatedanti-VCAM-1.Unboundbiotinylatedanti-VCAM-1andStreptavidin-HRPconjugateisremovedwithawashstepandHRPsubstratesolutionisaddedtothewells. ThepairofmonoclonalantibodiesusedinthisELISAdetectthesolubleformofVCAM-1presentinserum,plasma,urine,andotherBIOLOGicalfluids. Anamountofcoloredproductisformed,proportionaltotheamountofsolubleVCAM-1presentinthesample.Thereactionisterminatedbyadditionofacidandabsorbanceismeasuredat450nm.AstandardcurveispreparedfromsixsolubleVCAM-1standarddilutionsandtheVCAM-1sampleconcentrationisdetermined. Application: TheVCAM-1ELISAisanenzyme-linkedimmunosorbentassayforquantitativedetectionofsolubleVascularCellAdhesionMolecule-1incellculturesupernatants,serum,plasma,orotherbiologicalfluids.TheVCAM-1ELISAisforresearchuseonly.Notforuseindiagnosticprocedures.
AlternateNames	CD106
MaterialsRequiredbutNotDelivered	-5mLand10mLgraduatedPipettes -5μLto1000μLadjustablesinglechannelmicropipetteswith disposabletips -50μLto300μLadjustablemultichannelmicropipettewithdisposabletips -Multichannelmicropipettereservoir -Beakers,flasks,cylindersnecessaryforpreparationofreagents -Devicefordeliveryofwashsolution(multichannelwashbottleor automaticwashsystem) -MicrowellstripreadercapableofreADIngat450nm(620nmas optionalreferencewavelength) -Glass-distilledordeionizedwater -Statisticalcalculatorwithprogramtoperformlinearregressionanalysis.

ProductInformation
Components	·2aluminumpoucheswith3MicrowellStrips(16wellsperstrip)coatedwithMonoclonalAntibody(murine)tohumanVCAM-1 ·1vial(0.1mL)ConjugateMixturecontainingBiotin-ConjugatedAnti-VCAM-1Monoclonal(murine)AntibodymixedwithStreptavidin-HRP* ·2vials(200ng/mLasreconstituted)solubleVCAM-1Standard,lyophilized* ·1viallyophilizedcontrolserum ·1bottle(50mL)WashBufferConcentrate20X(PBSwith1%Tween20)* ·1vial(5mL)AssayBufferConcentrate20X(PBSwith1%Tween20and10%BSA)* ·1vial(7mL)SubstrateSolutionI(tetramethyl-benzidine) ·1vial(7mL)SubstrateSolutionII(0.02%bufferedhydrogenperoxide) ·1vial(12mL)StopSolution(1MPhosphoricAcid,H3PO4),readytouse ·1MicrowellStripHolder ·2AdhesivePlateCovers ·reagentscontaining0.01%thimerosalaspreservative
Detectionmethod	Chromogenic

ProductInformation

Components

·2aluminumpoucheswith3MicrowellStrips(16wellsperstrip)coatedwithMonoclonalAntibody(murine)tohumanVCAM-1
·1vial(0.1mL)ConjugateMixturecontainingBiotin-ConjugatedAnti-VCAM-1Monoclonal(murine)AntibodymixedwithStreptavidin-HRP*
·2vials(200ng/mLasreconstituted)solubleVCAM-1Standard,lyophilized*
·1viallyophilizedcontrolserum
·1bottle(50mL)WashBufferConcentrate20X(PBSwith1%Tween20)*
·1vial(5mL)AssayBufferConcentrate20X(PBSwith1%Tween20and10%BSA)*
·1vial(7mL)SubstrateSolutionI(tetramethyl-benzidine)
·1vial(7mL)SubstrateSolutionII(0.02%bufferedhydrogenperoxide)
·1vial(12mL)StopSolution(1MPhosphoricAcid,H3PO4),readytouse
·1MicrowellStripHolder
·2AdhesivePlateCovers
·reagentscontaining0.01%thimerosalaspreservative

Detectionmethod

Chromogenic

StorageandShippingInformation
StorageConditions	Precautions: ·Reagentsareintendedforresearchuseonlyandarenotforuseindiagnosticortherapeuticprocedures. ·Donotmixorsubstitutereagentswiththosefromotherlotsorothersources. ·Donotusekitreagentsbeyondexpirationdateonlabel. ·Donotexposekitreagentstostronglightduringstorageorincubation. ·Rubberordisposablelatexglovesshouldbewornwhilehandlingkitreagentsorspecimens. ·Somereagentscontainthimerosalaspreservative,whichishighlytoxicbyinhalation,ingestion,orcontactwithskin.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly. ·Avoidcontactofsubstratesolutionswithoxidizingagentsandmetal. ·Inordertoavoidmicrobialcontaminationorcross-contaminationofreagentsorspecimenswhichmayinvalidatethetestusedisposablepipettetipsand/orpipettes. ·Useclean,dedicatedreagenttraysfordispensingtheconjugateandsubstratereagents. ·Exposuretoacidswillinactivatetheconjugate. ·Glass-distilledwaterordeionizedwatermustbeusedforreagent preparation. ·Substratesolutionsmustbeatroomtemperaturepriortouse. ·Sinceexactconditionsmayvaryfromassaytoassay,astandard curvemustbeestablishedforeveryrun. ·Bacterialorfungalcontaminationofeitherscreensamplesorreagentsorcross-contaminationbetweenreagentsmaycauseerroneousresults. ·Disposablepipettetips,flasksorglasswarearepreferred.Reusableglasswaremustbewashedandthoroughlyrinsedofalldetergentsbeforeuse. ·Improperorinsufficientwashingatanystageoftheprocedurewillresultineitherfalsepositiveorfalsenegativeresults.CompletelyemptywellsbeforedispensingfreshWashBuffer,fillwithWashBufferasindicatedforeachwashcycleanddonotallowwellstosituncoveredordryforextendedperiods. ·Anyhumananti-mouseIgGantibody(HAMA)presentmayinterferewithassaysutilizingmurinemonoclonalantibodiesleadingtobothfalsepositiveandfalsenegativeresults.HAMAinterferencecanbereducedbyaddingmurineimmunoglobulins(serum,asciticfluid,ormonoclonalantibodiesofirrelevantspecificity)totheSampleDiluent.

StorageandShippingInformation

StorageConditions

Precautions:

·Reagentsareintendedforresearchuseonlyandarenotforuseindiagnosticortherapeuticprocedures.

·Donotmixorsubstitutereagentswiththosefromotherlotsorothersources.

·Donotusekitreagentsbeyondexpirationdateonlabel.

·Donotexposekitreagentstostronglightduringstorageorincubation.

·Rubberordisposablelatexglovesshouldbewornwhilehandlingkitreagentsorspecimens.

·Somereagentscontainthimerosalaspreservative,whichishighlytoxicbyinhalation,ingestion,orcontactwithskin.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly.

·Avoidcontactofsubstratesolutionswithoxidizingagentsandmetal.

·Inordertoavoidmicrobialcontaminationorcross-contaminationofreagentsorspecimenswhichmayinvalidatethetestusedisposablepipettetipsand/orpipettes.

·Useclean,dedicatedreagenttraysfordispensingtheconjugateandsubstratereagents.

·Exposuretoacidswillinactivatetheconjugate.

·Glass-distilledwaterordeionizedwatermustbeusedforreagent

preparation.

·Substratesolutionsmustbeatroomtemperaturepriortouse.

·Sinceexactconditionsmayvaryfromassaytoassay,astandard

curvemustbeestablishedforeveryrun.

·Bacterialorfungalcontaminationofeitherscreensamplesorreagentsorcross-contaminationbetweenreagentsmaycauseerroneousresults.

·Disposablepipettetips,flasksorglasswarearepreferred.Reusableglasswaremustbewashedandthoroughlyrinsedofalldetergentsbeforeuse.

·Improperorinsufficientwashingatanystageoftheprocedurewillresultineitherfalsepositiveorfalsenegativeresults.CompletelyemptywellsbeforedispensingfreshWashBuffer,fillwithWashBufferasindicatedforeachwashcycleanddonotallowwellstosituncoveredordryforextendedperiods.

·Anyhumananti-mouseIgGantibody(HAMA)presentmayinterferewithassaysutilizingmurinemonoclonalantibodiesleadingtobothfalsepositiveandfalsenegativeresults.HAMAinterferencecanbereducedbyaddingmurineimmunoglobulins(serum,asciticfluid,ormonoclonalantibodiesofirrelevantspecificity)totheSampleDiluent.

Applications
Application	ThisHumansVCAM-1ELISAisusedtomeasure&quantifysVCAM-1levelsinInflammation&Immunology&Neuroscienceresearch.
KeyApplications	ELISA

BiologicalInformation
SpeciesReactivity	Human
AnalytesAvailable	sVCAM-1
EntrezGeneNumber	NM_001078.2 NM_080682.1
EntrezGeneSummary	ThisgeneisamemberoftheIgsuperfamilyandencodesacellsurfacesialoglycoproteinexpressedbycytokine-activatedendothelium.ThistypeImembraneproteinmediatesleukocyte-endothelialcelladhesionandsignaltransduction,andmayplayaroleinthedevelopmentofartherosclerosisandrheumatoidarthritis.Twoalternativelysplicedtranscriptsencodingdifferentisoformshavebeendescribedforthisgene.
GeneSymbol	VCAM1 INCAM-100 MGC99561 CD106 DKFZp779G2333 L1CAM
UniProtNumber	P19320
UniProtSummary	FUNCTION:SwissProt:P19320#Importantincell-cellrecognition.Appearstofunctioninleukocyte-endothelialcelladhesion.Interactswiththebeta-1integrinVLA4onleukocytes,andmediatesbothadhesionandsignaltransduction.TheVCAM1/VLA4interactionmayplayapathophysiologicrolebothinimmuneresponsesandinleukocyteemigrationtositesofinflammation. SIZE:739aminoacids;81276Da SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. TISSUESPECIFICITY:Expressedoninflamedvascularendothelium,aswellasonmacrophage-likeanddendriticcelltypesinbothnormalandinflamedtissue. DOMAIN:SwissProt:P19320EitherthefirstorthefourthIg-likeC2-typedomainisrequiredforVLA4-dependentcelladhesion. PTM:Sialoglycoprotein. DISEASE:SwissProt:P19320#Mayplayanimportantroleinthegenesisofatherosclerosisandrheumatoidarthritis. SIMILARITY:Contains7Ig-likeC2-type(immunoglobulin-like)domains.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
￥830.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥830.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00
Parafilm M封口膜/PM996/4x125/整箱
￥1296.00

Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit

客戶服務

何為188

關注188微信公眾號