A water soluble alkyne dye for copper catalyzed Click Chemistry, an analog of Cy3® alkyne.
Sulfo-Cyanine3 is a fluorophore which is compatible with a wide range of fluorescent scanners, imagers, microscopes, and other instrumentation. It is a bright and photostable dye, which is also easily detected in gels by naked eye inlow amounts (<1 nmol).
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General properties
| Appearance: | dark red powder |
| Mass spec M+ increment: | 653.2 |
| Molecular weight: | 691.90 |
| Molecular formula: | C33H38KN3O7S2 |
| Solubility: | good solubility in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |