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Millipore/F1903 | Fibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified/F1903/500 µg

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貨號: F1903

品牌: Millipore

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Description
CatalogueNumber	F1903
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Fibronectin40kDaαChymotrypticFragment(Heparin-bindingregion),Humanpurified
Overview	Heparin-binding40kDafragmentofhumanplasmafibronectinpurifiedfromchymotrypticdigestofhumanplasmafibronectin.ThispreparationcontainstheCS-1heparin-bindingregionoffibronectin.Heparin-bindingfragmentpromotesCytoskeletonorganizationinintegrinsignaling.Thispreparationshowsonemajorbandat40kDainSDS-PAGEwithnohighermolecularweightspeciespresent.Smallerproteolyticfragmentsmaybepresent. HumansourcedrawmaterialusedintheproductionofthisproducttestednegativeforhepatitisBvirus,hepatitisCvirus(HCV),humanimmunodeficiencyvirustype-1(HIV-1)andtype-2(HIV-2),humanT-celllymphotropicvirus(HTLV-1andHTLV-2)andTreponemapallidum.Handleasifpotentiallyinfectious.

ProductInformation
Presentation	Purifiedbygelatin-Sepharose?andheparin-Sepharose?columnchromatography.Lyophilizedfromsodiumcitratebufferedsaline,20mM,NaCl150mM,pH7.4.Reconstitutewithdistilledwater.

StorageandShippingInformation
StorageConditions	Maintainlyophilizedmaterialat-20°Cforuptooneyearfromdateofreceipt.Afterreconstitution,maintainat-20°Cforuptosixmonthsinundilutedaliquots.Avoidrepeatedfreeze/thawcycles.

Applications
KeyApplications	CellCulture
ApplicationNotes	ELISA Affinitychromatography PossIBLyformolecularmappingoffibronectinfunction Cellattachmentandintegrinsignalingassays Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Concentration	1mg/mL
Source	Human
EntrezGeneNumber	NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1
EntrezGeneSummary	Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.
GeneSymbol	FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS FINC
UniProtNumber	P02751
UniProtSummary	FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains.
StemCellType	HumanEmbryonicStemCells MesenchymalStemCells NeuralStemCells HematopoieticStemCells EpithelialCells PancreaticStemCells InducedPluripotentStemCells

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Millipore/F1903 | Fibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified/F1903/500 µg

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