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Millipore/FC010-10MG | Human Plasma Fibronectin Purified Protein/FC010-10MG/10 mg10 x 1 mg

價格: ￥5604.00 市場價: 9340.00

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貨號: FC010-10MG

品牌: Millipore

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Description
CatalogueNumber	FC010-10MG
BrandFamily	Chemicon®
TradeName	Chemicon
Description	HumanPlasmaFibronectinPurifiedProtein
Overview	Humanfibronectin(HFN)issuitableforuseasanattachmentfactorinthepropagationofcellsinvitrowhenusedtocoatcellculturesurfaces,includingplasticware,glassware,andmicrocarrierbeads.

ProductInformation
Presentation	Suppliedin10individualvials.Liquidin150mMNaCl,10mMsodiumphosphate,pH7.5,sterilefiltered,containingnopreservatives.

StorageandShippingInformation
StorageConditions	Maintainat2-8°Cforupto6monthsfromdateofreceipt.Donotfreeze.

Applications
KeyApplications	CellCulture
ApplicationNotes	Immunochemicalstandard Cellattachmentandproliferationassaysonhumanendothelialcells,humankeratinocytesandhumandermalfibroblasts SuggestedProcedureforCoatingCellCultureware 1.DeterminetheamountofHFNneededtocoatculturevesselsbymultiplyingthetotalsurfacearea(cm2)bythedesiredconcentration(μg/mL)ofHFN.Recommendedamountis2-10μg/cm2. 2.ReconstituteHFNat1mg/mLanddilutetothedesiredconcentrationwithphysiologicalbuffer. 3.Wetthesurfaceofeachculturevesseltobecoatedwithaminimumamountofsterilebalancedsaltsolution(serumandproteinfree)requiredtocovertheentirearea. 4.IntroducetheproperCO2atmosphere,ifrequired. 5.AddthecalculatedamountofHFNtoeachculturevessel. 6.AllowHFNtoadsorbtothesurfaceofthevesselfor5-20minutes. 7.Removeresidualbalancedsaltsolutionbeforeproceedingwithstandardcellcultureprocedures

Applications

KeyApplications

CellCulture

ApplicationNotes

Immunochemicalstandard

Cellattachmentandproliferationassaysonhumanendothelialcells,humankeratinocytesandhumandermalfibroblasts

SuggestedProcedureforCoatingCellCultureware

1.DeterminetheamountofHFNneededtocoatculturevesselsbymultiplyingthetotalsurfacearea(cm2)bythedesiredconcentration(μg/mL)ofHFN.Recommendedamountis2-10μg/cm2.

2.ReconstituteHFNat1mg/mLanddilutetothedesiredconcentrationwithphysiologicalbuffer.

3.Wetthesurfaceofeachculturevesseltobecoatedwithaminimumamountofsterilebalancedsaltsolution(serumandproteinfree)requiredtocovertheentirearea.

4.IntroducetheproperCO2atmosphere,ifrequired.

5.AddthecalculatedamountofHFNtoeachculturevessel.

6.AllowHFNtoadsorbtothesurfaceofthevesselfor5-20minutes.

7.Removeresidualbalancedsaltsolutionbeforeproceedingwithstandardcellcultureprocedures

BIOLOGicalInformation
Concentration	1mg/mL
Purity	Approximately95%,asdeterminedbySDS-PAGE.Adoublebandof220kDaispresentunderreducedconditions.HFNispurifiedbyaffinitychromatographyongelatinagarose,followedbychromatographyonheparin-agarose.
Source	Humandonorplasma.
EntrezGeneNumber	NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1
EntrezGeneSummary	Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.
GeneSymbol	FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS FINC
UniProtNumber	P02751
UniProtSummary	FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains.
StemCellType	HumanEmbryonicStemCells MesenchymalStemCells NeuralStemCells HematopoieticStemCells EpithelialCells PancreaticStemCells InducedPluripotentStemCells

PhysicochemicalInformation

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Millipore/FC010-10MG | Human Plasma Fibronectin Purified Protein/FC010-10MG/10 mg10 x 1 mg

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