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Millipore/FCCH025142 | FlowCellect? Cell Cycle Checkpoint H2A.X DNA Damage Kit/FCCH025142/25 Tests / Kit

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貨號: FCCH025142

品牌: Millipore

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Description
CatalogueNumber	FCCH025142
TradeName	FlowCellect
Description	FlowCellect?CellCycleCheckpointH2A.XDNADamageKit
Overview	Millipore’sFlowCellect?DNADamage(HistoneH2A.X)andCellCycleAnalysisKitisdesignedtoidentifythelinkagebetweenDNAdamageinrelationtocellcyclecheckpointsinflowcytometryapplications.ThekitincludesadirectlyconjugatedAnti-phospho-HistoneH2A.X(Ser139)AlexaFluor?488antibodyplusaDNAdye(PropidiumIodide)whichallowstheresearchertoperformbivariateanalysistoidentifywhereDNAdamagetakesplacerelativetothepositioningwithinthecellcycle.Thekitincludesanoptimizedprotocolwithallofthenecessarycomponentstoprovidetheresearcheratrue“plugandplay”designfortheirresearchwhenstudyingDNAdamageandcellcyclecheckpoints.Thisimmunocytochemicalmethodisverysensitive,anditoffersanassaytoscreenanti-tumordrugsateveryphaseofthecellcycle. Inall,acomprehensiveunderstandingofDNAdamageanditsassociationwithcellcyclebehaviorcanprovidetheresearcherwithusefulinformationwhichwillbeimportantininterpretingtheintrinsicnatureofcellproliferation,apoptosis,DNArepair,andassistinthedevelopmentofanti-neoplasticagents.Millipore’sFlowCellect?DNADamage(HistoneH2A.X)andCellCycleAnalysisKitisdesignedtoallowtheresearchertoeasilyidentify,discriminate,andquantitatecellsundergoingDNAdamagerelativetoitspositioningwithinthecellcycle. AllFlowCellectkitsareoptimizedonguava?benchtopflowcytometers.FlowCellectkitscanbeusedonanyflowcytometerfollowingthesameprotocolprovidingresearchersareliableandfullyvalidatedsolutiontostudyDSBsandcellcycledistributionrightinthecomfortoftheirownlab.Thiskitcontainsoptimizedfixation,permeABIlization,wash,andflowbufferstoprovideresearcherswithacompletesolutionforbivariateanalysistoassessDNAdamageinrelationtopositioningwithinthecellcycle.
AlternateNames	FlowCellect
BackgroundInformation	DetectionofDNAdamageduetodoublestrandedbreaks(DSBs)isafundamentalmethodforassessingcellhealthandevaluationofanti-neoplasticagents.BivariateanalysismonitoringtheextentofH2A.XphosphorylationalongwithaDNAdyeoffersasensitiveandvaluabletoolinstudyingthefactorsthatinduceDNAdamage(measuredbythelevelofDSBs)and/oraffectDNArepair.UnderstandingwherethefirstindicationofDSBstakeplaceduringthecellcyclecanhelpresearchersunderstandthemechanismsinvolvedincellcyclearrestandDNArepairatcriticalcellcyclecheckpoints.MalfunctionsinthecellcycleinresponsetoDNAdamagecanincreasetheincidenceofcancersincetheDNArepairmachineryfailstooperateproperly.ThisisimportantbecausethecellhasseveralsystemsforinterruptingthecellcycleiftheDNArepairmachinerymalfunctions.EvidencesuggeststhatH2A.XphosphorylationmayrecruitcheckpointproteinstositesofDNAdamageandplayaroleinDNArepair. VariousfactorscancauseDNAdoublestrandedbreaksresultinginDNAdamage.PhysicalfactorssuchasUVandionizingrADIation,orchemicalfactorssuchassyntheticcompounds(anti-tumordrugs)canallcauseDNAdamage.RegardlessofthesourcetoinduceDNAdamage,theendresultwilleithercausecellcyclecheckpointarrest,apoptosis,orwilltriggertheDNArepairresponsetodamage.

ProductInformation
Components	1.20XAnti-p-HistoneH2A.X(Ser139),cloneJBW301-AlexaFluor?488Conjugate:(PartNo.CS206387)Onevialcontaining150μLantibody. 2.PropidiumIodideSolution:(PartNo.CS204836)Onevialcontaining400μLDNAdye. 3.RNaseAReagent:(PartNo.CS204821)Onevialcontaining25μL. 4.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mLbuffer. 5.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mLbuffer. 6.5XAssayBuffer:(PartNo.CS202124)Onebottlecontaining55mLbuffer. 7.1XPermeabilizationBuffer:(PartNo.CS203284)Twobottlescontaining14mLbuffer.
Detectionmethod	Fluorescent

ProductInformation

Components

1.20XAnti-p-HistoneH2A.X(Ser139),cloneJBW301-AlexaFluor?488Conjugate:(PartNo.CS206387)Onevialcontaining150μLantibody.
2.PropidiumIodideSolution:(PartNo.CS204836)Onevialcontaining400μLDNAdye.
3.RNaseAReagent:(PartNo.CS204821)Onevialcontaining25μL.
4.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mLbuffer.
5.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mLbuffer.
6.5XAssayBuffer:(PartNo.CS202124)Onebottlecontaining55mLbuffer.
7.1XPermeabilizationBuffer:(PartNo.CS203284)Twobottlescontaining14mLbuffer.

Detectionmethod

Fluorescent

StorageandShippingInformation
StorageConditions	4-8°Cforantibodiesandbuffers

Applications
Application	ThisFlowCellectDNADamage(HistoneH2A.X)&CellCycleAnalysisKitforFlowCytometryisdesignedtoidentifythelinkagebetweenDNAdamageinrelationtocellcyclecheckpointsinflowcytometryapplications.
KeyApplications	FlowCytometry

BIOLOGicalInformation
Clone	JBW301
Host	Mouse
SpeciesReactivity	Human
AntibodyType	MonoclonalAntibody

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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Millipore/FCCH025142 | FlowCellect? Cell Cycle Checkpoint H2A.X DNA Damage Kit/FCCH025142/25 Tests / Kit

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