當前位置： Millipore >>> Millipore/FCCS025106 | FlowCellect? MAPK Activation Dual Detection Kit/FCCS025106/25 Tests / Kit

Millipore/FCCS025106 | FlowCellect? MAPK Activation Dual Detection Kit/FCCS025106/25 Tests / Kit

價格: ￥4164.00 市場價: 6940.00

打印產品信息

貨號: FCCS025106

品牌: Millipore

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即購買加入購物車

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

Description
CatalogueNumber	FCCS025106
TradeName	FlowCellect
Description	FlowCellect?MAPKActivationDualDetectionKit
Overview	Millipore’sFlowCellect?MAPKActivationDualDetectionkitincludestwodirectlyconjugatedantibodies,aphospho-specificAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187)-PEandanAnti-ERK1/2-AlexaFluor?647conjugatedantibodytomeasuretotallevelsofERK.ThistwocolorflowcytometrykitisdesignedtomeasuretheextentofMAPKphosphorylationrelativetothetotalMAPKexpressioninanygivencellpopulation.Bydoingsuch,thelevelsofboththetotalandphosphorylatedproteincanbemeasuredsimultaneouslyinthesamecell,resultinginanormalizedandaccuratemeasurementofMAPKactivationafterstimulation.Moreover,simultaneousmeasurementofbothtotalandphospho-ERK1/2confirmstargetspecificityofthephosphorylationevent.Together,atotalandphosphoantibodyduoperformedinmultiplexprovidesanenhancedandmorereliabledetectionofthephospho:totalratiowithinamixedpopulation. AllFlowCellectkitsaresuppliedwithoptimizedfixation,permeABIlization,wash,andassaybufferstoprovideresearcherstheabilitytodetecttheactivationofERKinflowapplications.Sufficientreagentsareprovidedfor25two-colorsamples.Thekitincludesalloptimizedfluorescentlylabeledantibodiesandbuffersnecessaryforcellpreparationandanalysis.
AlternateNames	FlowCellect
BackgroundInformation	Erk(Extracellularsignal-RelatedKinase)isafamilyoftwo,highlyhomologousproteinsdenotedasErk1(p44,MAPK3)andErk2(p42,MAPK1)thatbothfunctioninthesamepathway.ThetwoproteinsareoftenreferredtocollectivelyasErk1/2orp44/p42MAPkinase.TheErkpathwayisconsideredtheclassical,canonicalMAPK(Mitogen-ActivatedProteinKinase)signalingpathway.Itisanevolutionarilyconservedpathwaythatcontrolsandisacriticalregulatorofthegrowthandsurvivalthroughthepromotionofcellproliferationandthepreventionofapoptosis.Erkisinvolvedinthecontrolofmanyfundamentalcellularprocessesincludingcellproliferation,survival,differentiation,apoptosis,motilityandmetabolism.Erkisactivatedbygrowthfactorstimulationofreceptortyrosinekinases(RTKs),GPCR,and/orintegrinstimulation.ThisactivatestheRas-Raf-MEK-Erkpathwaythatresultsinthephosphorylation/activationofErk1/2(p44/p42)ontheTxYmotif(Thr202/Tyr204andThr185/Tyr187forErk1&Erk2,respectively). Theregulationofalargenumberofcellularprocesses(suchascellproliferation/differentiation,cellsurvivalandapoptosis)isdependentontheactivationofMAPKsignalingpathway,whichcanbemeasuredbythephosphorylationofERK1/2.

ProductInformation
Components	1.20XAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187)PEConjugatedAntibody:(PartNo.CS203329)Onevialcontaining150μL. 2.20XAnti-ERK1/2-AlexaFluor?647ConjugatedAntibody:(PartNo.CS205075)Onevialcontaining150μL. 3.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mL. 4.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mL. 5.5XAssayBuffer:(PartNo.CS202124)One55mLbottle 6.1XPermeabilizationBuffer:(PartNo.CS203284)Onebottlecontaining13mL.
Detectionmethod	Fluorescent

ProductInformation

Components

1.20XAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187)PEConjugatedAntibody:(PartNo.CS203329)Onevialcontaining150μL.
2.20XAnti-ERK1/2-AlexaFluor?647ConjugatedAntibody:(PartNo.CS205075)Onevialcontaining150μL.
3.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mL.
4.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mL.
5.5XAssayBuffer:(PartNo.CS202124)One55mLbottle
6.1XPermeabilizationBuffer:(PartNo.CS203284)Onebottlecontaining13mL.

Detectionmethod

Fluorescent

StorageandShippingInformation
StorageConditions	4-8°Cforantibodiesandbuffers

Applications
Application	ThisFlowCellectMAPKActivationDualDetectionKitforFlowCytometryincludes2conjugatedantibodies,phospho-specificAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187)-PE&Anti-ERK1/2-AlexaFluor647tomeasuretotallevelsofERK.
KeyApplications	FlowCytometry

BIOLOGicalInformation
Host	Mouse
SpeciesReactivity	Human
AntibodyType	MonoclonalAntibody

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Parafilm M封口膜/PM996/4x125/盒
￥0.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥850.00
ThermoFisherQ32856Qubit? Assay Tubes現貨
￥590.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00

Millipore/FCCS025106 | FlowCellect? MAPK Activation Dual Detection Kit/FCCS025106/25 Tests / Kit

客戶服務

何為188

關注188微信公眾號