Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique.
Quality Control Testing:
Representative images of human cell stain with the dual color FISH probe. The left image is two copies of CCND1 gene and two copies of chromosome 11 in HL60S cell line; and the right image is two copies of chromosome 11 and higher copy number of CCND1 gene (amplification) in SKMEL2 cell line.
Storage Instruction:
Store at -20°C in the dark.
Note:
Hybridization position of the probes on the chromosome.
Probe 1:Size:Fluorophore:Location:
CCND1Approximately 162kbOrange11q13
Probe 2:Size:Fluorophore:Location:
CEN11qApproximately 0.68kbGreen11p11.11-q11
Origin:
Human
Source:
Genomic DNA
Regulation Status:
For research use only (RUO)
Datasheet:
Download
Publication Reference
1.
Detection of CCND1 Locus Amplification by Fluorescence In Situ Hybridization.Balázs M, Koroknai V, Szász I, Ecsedi S.Methods Mol Biol. 2018;1726:85-100.
2.
Integrated analysis of pediatric glioblastoma reveals a subset of biologically favorable tumors with associated molecular prognostic markers.Korshunov A, Ryzhova M, Hovestadt V, Bender S, Sturm D, Capper D, Meyer J, Schrimpf D, Kool M, Northcott PA, Zheludkova O, Milde T, Witt O, Kulozik AE, Reifenberger G, Jabado N, Perry A, Lichter P, von Deimling A, Pfister SM, Jones DT.Acta Neuropathol. 2015 May;129(5):669-78. Epub 2015 Mar 10.
Applications
Fluorescent In Situ Hybridization
Protocol Download
Application Image
Fluorescent In Situ Hybridization
Gene Information
Entrez GeneID:
595
Gene Name:
CCND1
Gene Alias:
BCL1,D11S287E,PRAD1,U21B31
Gene Description:
cyclin D1
Omim ID:
151400, 168461, 193300, 254500
Gene Ontology:
Hyperlink
Gene Summary:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq
Other Designations:
B-cell CLL/lymphoma 1,G1/S-specific cyclin D1
Gene Pathway
Acute myeloid leukemia
Bladder cancer
Cell cycle
Chronic myeloid leukemia
Colorectal cancer
Endometrial cancer
Focal adhesion
Glioma
Jak-STAT signaling pathway
Melanoma
Non-small cell lung cancer
p53 signaling pathway
Pancreatic cancer
Pathways in cancer
Prostate cancer
Small cell lung cancer
Thyroid cancer
Wnt signaling pathway
Related Disease
Adenocarcinoma
Adenocarcinoma, Mucinous
Adenoma
Ataxia telangiectasia
Barrett Esophagus
Brain Neoplasms
Breast cancer
Breast Neoplasms
Breast Neoplasms, Male
Carcinoma
Carcinoma in Situ
Carcinoma, Basal Cell
Carcinoma, Hepatocellular
Carcinoma, Non-Small-Cell Lung
Carcinoma, Renal Cell
Carcinoma, Squamous Cell
Carcinoma, Transitional Cell
Cell Transformation, Neoplastic
Cell Transformation, Viral
... see more
Chromosome Aberrations
Chronic Disease
Colitis, Ulcerative
Colon cancer
Colorectal Neoplasms
Colorectal Neoplasms, Hereditary Nonpolyposis
Crohn Disease
Disease Progression
Disease Susceptibility
DNA Damage
Endometrial Neoplasms
Esophageal Neoplasms
Fetal Diseases
Gastritis
Gastroesophageal Reflux
Genetic Predisposition to Disease
Genomic Instability
Glioma
Head and Neck Neoplasms
Helicobacter Infections
Hematologic Diseases
Hemophilia A
Hemophilia A
Hepatoblastoma
Hodgkin Disease
Hyperparathyroidism
Hypopharyngeal Neoplasms
Inflammatory Bowel Diseases
Insulin Resistance
Intestinal Neoplasms
Kidney Failure, Chronic
Kidney Neoplasms
Laryngeal Diseases
Laryngeal Neoplasms
Leiomyoma
Leukemia, Lymphocytic, Acute
Leukemia, Lymphocytic, Acute, L1
Leukemia, Lymphocytic, Chronic
Leukoplakia, Oral
Liver Neoplasms
Lung Neoplasms
Lymphatic Metastasis
Lymphoma, B-Cell
Lymphoma, Follicular
Lymphoma, Large B-Cell, Diffuse
Lymphoma, Non-Hodgkin
Lymphoproliferative Disorders
Meningeal Neoplasms
Meningioma
Mouth Neoplasms
Nasopharyngeal Neoplasms
Neoplasm Invasiveness
Neoplasm Metastasis
Neoplasm Recurrence, Local
Neoplasms
Neoplasms, Ductal, Lobular, and Medullary
Neoplasms, Glandular and Epithelial
Neoplasms, Radiation-Induced
Neuroma, Acoustic
Obesity
Occupational Diseases
Oropharyngeal Neoplasms
Ovarian cancer
Ovarian Neoplasms
Papillomavirus Infections
Peptic Ulcer
Pituitary Neoplasms
Precancerous Conditions
Prolactinoma
Prostate cancer
Prostatic Hyperplasia
Prostatic Neoplasms
Pulmonary Disease, Chronic Obstructive
Rectal Neoplasms
Recurrence
Retinoblastoma
Skin Neoplasms
Stomach Neoplasms
Translocation, Genetic
Urinary Bladder Neoplasms
Uterine Cervical Neoplasms
Uterine Neoplasms
Waldenstrom Macroglobulinemia
Werner syndrome
... hidden
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