mutaFISH™ EGFR T790M T790wt RNA Probes is designed to detect human EGFR T790M gene mutation on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
Probe Position:
Regulatory Status:
For research use only (RUO)
Datasheet:
Download
Video:
Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human H1975 cells captured by CytoQuest™ CR. EGFR T790M point mutation was detected via orange signal (Cy3), and EGFR T790wt was detected via red signal (Cy5).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations:
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor
Interactome
Gene Pathway
Adherens junction
Bladder cancer
Calcium signaling pathway
Colorectal cancer
Cytokine-cytokine receptor interaction
Dorso-ventral axis formation
Endocytosis
Endometrial cancer
Epithelial cell signaling in Helicobacter pylori infection
ErbB signaling pathway
Focal adhesion
Gap junction
Glioma
GnRH signaling pathway
MAPK signaling pathway
Melanoma
Non-small cell lung cancer
Pancreatic cancer
Pathways in cancer
Prostate cancer
Regulation of actin cytoskeleton
Related Disease
Adenocarcinoma
Adenocarcinoma, Bronchiolo-Alveolar
Adenocarcinoma, Mucinous
Adenocarcinoma, Papillary
Anus Neoplasms
Asthma
Asthma
Astrocytoma
Atherosclerosis
Barrett Esophagus
Bile Duct Neoplasms
Biliary Tract Neoplasms
Bipolar Disorder
Brain Neoplasms
Breast cancer
Breast Neoplasms
Bronchial Hyperreactivity
Carcinoma
Carcinoma, Adenosquamous
... see more
Carcinoma, Giant Cell
Carcinoma, Hepatocellular
Carcinoma, Large Cell
Carcinoma, Non-Small-Cell Lung
Carcinoma, Papillary
Carcinoma, Renal Cell
Carcinoma, Small Cell
Carcinoma, Squamous Cell
Cardiomyopathy, Dilated
Cardiovascular Diseases
Cell Transformation, Neoplastic
Central Nervous System Neoplasms
Cervical Intraepithelial Neoplasia
Cholangiocarcinoma
Chromosome Aberrations
Chromosome Deletion
Cleft Lip
Cleft Palate
Cocarcinogenesis
Colon cancer
Colonic Neoplasms
Colorectal Neoplasms
Cystadenocarcinoma, Mucinous
Cystadenocarcinoma, Serous
Diabetes Mellitus, Type 2
Diarrhea
Disease Progression
Disease Susceptibility
DNA Damage
Drug Eruptions
Drug Toxicity
Edema
Endometrial Neoplasms
Endometriosis
Esophageal Neoplasms
Exanthema
Genetic Diseases, Inborn
Genetic Predisposition to Disease
Glioblastoma
Glioma
Head and Neck Neoplasms
Hepatitis C
HIV Infections
Hyperparathyroidism, Secondary
Hypersensitivity
Hypopharyngeal Neoplasms
Kidney Failure, Chronic
Kidney Neoplasms
Liver Diseases
Liver Neoplasms
Lung carcinoma, small cell
Lung Neoplasms
Lupus Erythematosus, Systemic
Lymphatic Metastasis
Mental Disorders
Mouth Neoplasms
Myoma
Nasopharyngeal Neoplasms
Neoplasm Invasiveness
Neoplasm Metastasis
Neoplasm Recurrence, Local
Neoplasms
Neoplasms, Glandular and Epithelial
Neoplasms, Radiation-Induced
Neoplasms, Second Primary
Neoplasms, Squamous Cell
Osteosarcoma
Otorhinolaryngologic Neoplasms
Ovarian cancer
Ovarian Neoplasms
Pancreatic cancer
Pancreatic Neoplasms
Papillomavirus Infections
Polycystic kidney disease
Polycystic Kidney, Autosomal Dominant
Precancerous Conditions
Prostate cancer
Prostatic Hyperplasia
Prostatic Neoplasms
Pulmonary Disease, Chronic Obstructive
Ras oncogene
Rectal Neoplasms
Recurrence
Skin Neoplasms
Small Cell Lung Carcinoma
Stomach Neoplasms
Thyroid Neoplasms
Tongue Neoplasms
Tonsillar Neoplasms
Urinary Bladder Neoplasms
Urinary Calculi
Uterine Cervical Neoplasms
Uterine Neoplasms
Viremia
Werner syndrome
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