Properties:
| RelatedCategories | Analytical/Chromatography,GEHealthcareLifeSciencesProteinSamplePreparationFormats,HiTrap&PD-10Cartridges,HiTrapSystem/syringe,MolecularBIOLOGy, | 
| shelflife | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. | 
| packaging | packof5×5 mL | 
| mfr.no. | GEHealthcare,17-0921-04 | 
| parameter | 42psi | 
| bedvolume | 5 mL | 
| columnI.D. | 16 mm | 
| matrix | 6%cross-linkedagarose | 
| particlesize | 45-165 μm | 
| averagediameter | 90 μm | 
| cleaning | 2-14(Mediumunchargedwithmetalions.) | 
| workingrange | 3-12 | 
| capacity | ~40 mgbindingcapacity(histidine-taggedprotein/mlmediumwhenchangedwithNi2+)(Protein-andmetal-iondependent.) | 
| suitABIlity | suitableforbioprocessmedium | 
Description:
AnalysisNote
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LegalInformation
IMACSepharoseproducts,NiSepharoseproductsandFeSepharoseproducts
TheseproductsaresoldunderalicensefromSigma-AldrichunderpatentnumberEP1276716(Metalchelatingcompositions)andequivalentpatentsandpatentapplicationsinothercountries.
HiPrepisatrademarkofGEHealthcare
HiScreenisatrademarkofGEHealthcare
HiTrapisaregisteredtrademarkofGEHealthcare
SepharoseisaregisteredtrademarkofGEHealthcare
Application
IMACSepharose®FFissuppliedfreeofmetalions.Itischargedbytheuserwiththetransitionmetalionofchoice(e.g.Cu2+,Zn2+,Ni2+,orCo2+);thesemetalionswillbindtothecovalentlyimmobilizedchelatingligandontheSepharose.
IMACSepharose®6FastFLowconsistsof90?mbeadsofhighlycross-linkedAgarosetowhichachelatinggrouphasbeencovalentlycoupled.Thischelatinggroupcanbechargedwithsuitablemetalions,specificforyourtargetProteinofinterest.ThebeadsizeofIMACSepharose®6FastFLowenableshighflowproperties,makingthemediumanexcellentchoiceforscalinguppurificationatlaboratoryscaleandpurificationofhistidine-taggedProteinsatprocessscale.LowmetalionleakagemeansthattheactivityofthepurifiedProteinisretainedandtheriskofprecipitationreduced,whichresultsinincreasedpurity,activity,andyieldofthetargetProtein.LeakageofmetalionsintotheelutedtargetProteinpoolfromIMACSepharose®6FastFLowisgenerallyLowundernormalconditions.
IMACSepharose®6FastFLowishighlystableandcompatIBLewithawiderangeofcommonadditives.Thishelpsmaintainbiologicalactivityandincreaseproductyield,whileatthesametimegreatlyexpandingtherangeofsuitableoperatingconditions.
ThemediumisavailableprepackedinHiTrap®HiScreen™andHiPrep™columnformatsforconvenientpurificationofhistidine-taggedProteinsatlaboratoryscale.Initialpurificationusinga1mLHiTrap®IMACFFcolumncanbescaledupfurtherwiththe5mLcolumn.Purificationcanbescaledupevenmoreusing20mLHiPrep™IMACFF16/10columns.PrepackedHiScreen™IMACFFwitha10cmbedheightistheformatofchoiceformethodoptimizationandprocessdevelopment.
FeaturesandBenefits
• Foroptimizingpurificationofhistidine-taggedProteinswhenNi2+isnotthebestchoiceofmetalion.
• Convenientlychargewithyourmetalofchoice.
• Optimalbeadsizeforscalingup.
• Highbindingcapacity.
Generaldescription
HiTrap®IMACFFisprepackedwithIMACSepharose®6FastFlow.ThemediumischargedwiththemetalionofyourchoiceforImmobilizedMetalionAffinityChromatography(IMAC)andsubsequentpurificationofpolyhistidinetaggedproteins.
Safety:
Symbol 

  GHS02
Precautionarystatements 
P210-P370+P378
RIDADR 
UN1170-class3-PG3-Ethanol,solution