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特別 提示
| Related Categories | Analytical/Chromatography, GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap & PD-10 Cartridges, HiTrap System/syringe, Molecular Biology, |
| shelf life | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| packaging | pack of 5 × 5 mL |
| mfr. no. | GE Healthcare, 17-5080-02 |
| parameter | <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.) |
| 42 psi | |
| bed size | 16 mm × 25 mm |
| bed volume | 5 mL |
To view the Certificate of Analysis for this product, please visit www.gelifesciences.com
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• Quick, convenient preparative purification of monoclonal antibodies.
• Well-established protein A-based medium used in many approved MAb processes.
• Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.
HiTrap® rProtein A FF columns are prepacked with rProtein A Sepharose® Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose® Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
HiTrap is a registered trademark of GE Healthcare
Sepharose is a registered trademark of GE Healthcare
GHS02
