Properties:
| Related Categories |
Analytical/Chromatography,
GE Healthcare Life Sciences Protein Sample Preparation Formats,
HiTrap & PD-10 Cartridges,
HiTrap System/syringe,
Molecular Biology,
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shelf life
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Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
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packaging
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pack of 1 × 5 mL
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mfr. no.
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GE Healthcare, 17-5111-01
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parameter
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<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
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42 psi
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bed size
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16 mm × 25 mm
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bed volume
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5 mL
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column I.D.
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16 mm
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matrix
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highly cross-linked 6% agarose
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avg. part. size
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34 μm
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cleaning
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2 - 13
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working range
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3 - 11
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storage temp.
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2-8°C
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Description:
Analysis Note
www.gelifesciences.com
.
Application
HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose® High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.
Features and Benefits
• Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
• Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose® High Performance.
• Recovery and purity of IgY are excellent.
• Can be operated with a liquid chromatography system such as ?KTA® design.
• The top and bottom frits are manufactured from porous polyethylene
• Connectors for usage with different chromatography systems and other equipment are included
General description
HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose® High Performance for fast and easy purification of IgY from egg yolk.
Legal Information
HiTrap is a registered trademark of GE Healthcare
Sepharose is a registered trademark of GE Healthcare
?KTA is a registered trademark of GE Healthcare
Safety:
Symbol

GHS02
Precautionary statements
P210-P370 + P378
RIDADR
UN1170 - class 3 - PG 3 - Ethanol, solution