Properties:
| Related Categories |
Analytical/Chromatography,
GE Healthcare Life Sciences Protein Sample Preparation Formats,
HiTrap & PD-10 Cartridges,
HiTrap System/syringe,
Molecular Biology,
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|
shelf life
|
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
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packaging
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pack of 2 × 1 mL
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mfr. no.
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GE Healthcare, 17-5143-02
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parameter
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42 psi
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bed size
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7 mm × 25 mm
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bed volume
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1 mL
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column I.D.
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7 mm
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matrix
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highly cross-linked 4% agarose
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average diameter
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90 μm
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cleaning
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1 - 9
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working range
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2 - 8
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storage temp.
|
2-8°C
|
Description:
Analysis Note
www.gelifesciences.com
.
Application
HiTrap® Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
The medium, Benzamidine Sepharose® 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ?KTAdesign or FPLC™ System.
Features and Benefits
• Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
• Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
• HiTrap® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
• High binding capacity.
• The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ?KTA® design or FPLC™ System.
General description
HiTrap® Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose® 4 Fast FLow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.
Legal Information
FPLC is a trademark of GE Healthcare
HiTrap is a registered trademark of GE Healthcare
Sepharose is a registered trademark of GE Healthcare
?KTA is a registered trademark of GE Healthcare
Safety:
Symbol

GHS02
Precautionary statements
P210-P370 + P378
RIDADR
UN1170 - class 3 - PG 3 - Ethanol, solution