Properties:
| RelatedCategories | Analytical/Chromatography,GEHealthcareLifeSciencesProteinSamplePreparationFormats,HiTrap&PD-10Cartridges,HiTrapSystem/syringe,MolecularBIOLOGy, |
| shelflife | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. |
| packaging | packof5×5 mL |
| mfr.no. | GEHealthcare,28-9537-67 |
| parameter | 1ml/min-4ml/minflowrate(Thepressureoverthepackedbedvariesdependingonarangeofparameterssuchasthecharacteristicsofthechromatographymediumandthecolumntubingused.) |
| | 3bar |
| columnI.D. | 7 mm |
| matrix | 6%cross-linkedagarose |
| particlesize | 60-160 μm |
| cleaning | 2-14(Co2+-strippedmedium) |
| workingrange | 3-12(Co2+-strippedmedium) |
| capacity | ≥20 mgbindingcapacity(histidine-taggedprotein)(Thepressureoverthepackedbedvariesdependingonarangeofparameterssuchasthecharacteristicsofthechromatographymediumandthecolumntubingused.) |
| storagetemp. | 2-8°C |
Description:
AnalysisNote
ToviewtheCertificateofAnalysisforthisproduct,pleasevisitwww.geLifesciences.com
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FeaturesandBenefits
• Columnsprepackedwithcobalt-basedchromatographymediadesignedforpurificationofhistidine-taggedrecombinantproteinsbyimmobilizedmetalaffinitychromatography(IMAC).
• SuitableforIMACpurificationofhistidine-taggedproteinswhenNi2+isnottheoptimalchoiceofmetalion.
• CompatIBLewithcommonlyusedIMACreagentsandappropriateforpurifyingproteinsundernativeordenaturingconditions.
• PrepackedHiTrap®Talon®crudecolumnssavetimebyallowingtheapplicationofunclarifiedsamplesdirectly,minimizinglaboranddegradationofproteins.
• HiTrap®Talon®crudecolumnsallowrepeatedrunswithoutstripping,cleaning,orCo2+recharging.
Generaldescription
HiTrap®Talon®crude1mLand5mLcolumnsallowforrapidseparationsandeasyscale-upwithaminimumofsamplepreparationandequipment.
Talon®Superflowisacobalt-basedIMACmediumofferingdifferentselectivitycomparedtonickel-chargedmedia.Talon®SuperflowandprepackedHiTrap®Talon®crudecolumnsallowproteinpurificationundernativeordenaturingconditionsandcanbeusedwithprokaryoticandeukaryoticexpressionsystems.
HiTrap®Talon®crudecolumnsallowforrapidseparationsandeasyscale-up.Samplescanbeapplieddirectlytothecolumnafterthoroughcelldisruption-withoutprecentrifugationandfiltration.ThefiltersinthetopandbottomofthecolumnmakeitpossibletodirectlyloadunclarifiedlysatesonthecolumnwithoutcausingbackpressureproblemsorleakageoftheTalon®Superflowbeads.PriortoloADIngsamplesonHiTrap®Talon®crudecolumns,celllysateshouldbepreparedusingmethodssuchassonication,homogenization,orfreeze/thaw.
LegalInformation
HiTrapisaregisteredtrademarkofGEHealthcare
TalonisaregisteredtrademarkofClontech
Safety:
Precautionarystatements
P210-P235-P241-P280-P303+P361+P353-P501
RIDADR
UN1170-class3-PG3-Ethanol,solution