Properties:
Related Categories |
Analytical/Chromatography,
GE Life Sciences Gel Filtration Columns,
Gel Filtration Chromatography,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations,
|
material
|
Bed Support (polyamide)
|
|
borosilicate glass column
|
|
polypropylene fiber (reinforced glass)
|
shelf life
|
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
|
mol wt
|
mol wt <10000 Da (globular proteins)
|
packaging
|
pkg of 1 ea
|
mfr. no.
|
GE Healthcare, 28-9893-31
|
parameter
|
<1.0 mL/min flow rate
|
|
0.3 Mpa (42 psi)
|
bed volume
|
120 mL
|
column L × I.D.
|
60 cm × 16 mm
|
sample volume
|
<5 mL
|
matrix
|
agarose dextran composite
|
|
dextran-agarose particle platform
|
matrix active group
|
dextran-agarose phase
|
|
dextran-agarose phase
|
avg. part. size
|
34 μm
|
cleaning
|
1 - 14
|
working range
|
3 - 12
|
mode of chromatography
|
Size exclusion (SEC)
|
Description:
Analysis Note
www.gelifesciences.com
.
Application
Superdex® prep grade (pg) is produced by covalent bonding of Dextran to highly cross-linked Agarose.The separation properties of these media are determined by the Dextran component. Steep selectivity curves give excellent resolving power for peptides and Proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Features and Benefits
• High resolution with short run times and good recovery.
• High capacity at high flow rates.
• Separations can be scaled up to production levels.
• Prepacked for convenience and reproducibility.
• 1/16” fittings for convenient connections to ?KTA® design systems.
General description
HiLoad® 16/600 Superdex® 30 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography separating peptides.
Recommended products
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Suitability
All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating peptides
Legal Information
HiLoad is a registered trademark of GE Healthcare
Superdex is a registered trademark of GE Healthcare
?KTA is a registered trademark of GE Healthcare
Safety:
RIDADR
NONH for all modes of transport