Properties:
| RelatedCategories | Analytical/Chromatography,GEDesaltingColumns,GelFiltrationChromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations, |
| shelflife | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. |
| packaging | packof5 mL |
| mfr.no. | GEHealthcare,29-0486-84 |
| availABIlity | notavailableinNorthAmerica |
| storagecondition | drymedia |
| parameter | <15mL/minflowrate(Thepressureoverthepackedbedvariesdependingonarangeofparameterssuchasthecharacteristicsofthechromatographymediumandthecolumntubingused.) |
| | 42psi |
| bedsize | 16 mm×25 mm |
| bedvolume | 5 mL |
| columnI.D. | 16 mm |
| matrix | cross-linkeddextran |
| particlesize | >20 μm(dry) |
| cleaning | 2-13 |
| workingrange | 2-13 |
| suitability | suitableforbioprocessmedium |
Description:
Application
Gelfiltrationhassuccessfullybeenemployedforsizebasedseparationsofmacromoleculessincethelate1950s.Gelfiltration,orsizeexclusionchromatography,maybeperformedinthreeprincipallydifferentmodes,dependingonthesizedifferencesofthesolutestobeseparated(i)groupseparation(ii)fractionation(iii)determinationofmolecularmass(distributions)Whenthesizedifferenceislarge,i.e.afactorofmorethan10,wetalkaboutgroupseparation(e.g.desalting,bufferexchange)andwhenthesizedifferenceissmall,i.e.afactorof2to5,wetalkaboutfractionation.Thethirdmodeisanalyticalgelfiltration,e.g.determinationofmolecularmassdistributions,whereanarrayofmolecularmassesistobeseparated.Thesedifferentmodesputdifferentrequirementsontheequipmentandmediausedandwillyieldwidelydifferentproductivityintermsofinformationormaterialprocessed.
FeaturesandBenefits
• Handlessamplevolumesof1.5mL,orlargervolumesbyconnectinguptofivecolumnsinseries.
• Replacesdialysisasthemethodofchoicefordesalting.
• DesaltingrequireslessthanoneminuteduetothehighrigidityofSephadexG-25andtheoptimizedpackingtechnology.
Generaldescription
HiTrapDesaltingisaprepacked,readytousecolumnforgroupseparationbetweenhighandlowmolecularweightsubstances,i.e.,bufferexchangepriortoorafterdifferentchromatographicsteps,removaloflowmolecularweightcontaminantsorremovalofreagentstoterminateareaction.
LegalInformation
HiTrapisaregisteredtrademarkofGEHealthcare
Safety:
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