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Millipore/HT17MG-14K-PX25 | MILLIPLEX <small>MAP</small> Human TH17 Magnetic Bead Panel Premixed - 25 Plex - Immunology

Millipore/HT17MG-14K-PX25 | MILLIPLEX MAP Human TH17 Magnetic Bead Panel Premixed - 25 Plex - Immunology

價格: ￥46848.00 市場價: 78080.00

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貨號: HT17MG-14K-PX25

品牌: Millipore

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Description
CatalogueNumber	HT17MG-14K-PX25
TradeName	MILLIPLEX
Description	MILLIPLEXMAPHumanTH17MagneticBeadPanelPremixed-25Plex-ImmunologyMultiplexAssay
Overview	EMDMillipore’sMILLIPLEX?MAPHumanTH17PremixedMagneticBeadPanelisa25-plexkittobeusedforthesimultaneousquantificationofthefollowinganalytesinserum,plasmaorculturesupernatantsamples:GM-CSF,IFN-γ,IL-1β,IL-2,IL-4,IL-5,IL-6,IL-9,IL-10,IL-12(p70),IL-13,IL-15,IL-17A,IL-17F,IL-17E/IL-25,IL-21,IL-22,IL-23,IL-27,IL-28A,IL-31,IL-33,MIP-3α/CCL20,TNF-αandTNFβ. MILLIPLEX?MAPoffersthebroadestselectionofanalytesacrossawiderangeofdiseasestatesandspecies.Oncetheanalytesofinteresthavebeenidentified,youcanrelyonthequalitythatwebuildintoeachkittoproduceresultsyoucantrust.Inadditiontotheassaycharacteristicslistedintheprotocol,otherperformancecriteriaevaluatedduringthevalidationprocessinclude:cross-reactivity,dilutionlinearity,kitstABIlityandsamplebehavior(e.g.detectabilityandstability).Eachpanelandkitmeetsstringentmanufacturingcriteriatoensurebatch-to-batchreproducibility. EachMILLIPLEX?MAPpanelandkitincludes: ?Qualitycontrols(QCs)providedtoqualifyassayperformance ?Comparisonofstandard(calibrator)andQClotstoareferencelottoensurelot-to-lotconsistency ?Optimizedserummatrixtomimicnativeanalyteenvironment ?Detectionantibodycocktailsdesignedtoyieldconsistentanalyteprofileswithinpanel MILLIPLEX?MAPoffersyou: oTheabilitytochooseanycombinationofanalytesfromourpanelofsevenanalytestodesignacustomkitthatbettermeetsyourneeds. oAconvenient“all-in-one”boxformatthatgivesyoutheassurancethatyouwillhaveallthenecessaryreagentsyouneedtorunyourassay. EMDMillipore’sMILLIPLEX?MAPHumanTH17MagneticBeadPremixedPanelispartofthemostversatilesystemavailableforcytokineresearch.FromoursingletomultiplexbioMarkersolutions,wepartnerwithyoutodesign,develop,analyticallyvalidateandbuildthemostcomprehensivelibraryavailableforproteindetectionandquantitation.
BackgroundInformation	CD4T-helpercellsaremajorplayersinadaptiveimmunity.Basedontheirexpressionprofileoftranscriptionfactorsandsecretedcytokines,thesecellsaredividedintothemajorsubsetsTh1,Th2,Th17andTregulatory(Treg)cells.Th1cells,inducedbyIL-12andenhancedbyIFNγ,secreteIFNγthatactivatesmacrophages.InducedbyIL-4,Th2cellsprimarilyproduceIL-4,IL-5,IL-13,IL-25andIL-17E,playingaroleinallergy.Tregcells,inducedbyTGFβ,producetheanti-inflammatorycytokinesIL-10andTGFβandfunctiontocontrolT-cellresponsestopreventautoimmunereactions.Th17cells,amorerecentlydiscoveredsubsetcharacterizedbytheproductionofIL-17,areinducedbyTGFβcombinedwithIL-23andIL-1βinhumans,andTGFβ,IL-6andIL-21inmice.ActivatedTh17cellssecreteIL-17A,IL-17F,IL-21,IL-22andTNFαtopromotetissueinflammation. Th17cellsareinvolvedintheclearanceofextracellularbacteriaandfungi.TheyareabundantintheintestinallaminapropriaandfunctionasabarrieragainstinvADIngpathogens.ExcessiveamountsofTh17cellshavebeenimplicatedinthepathogenesisofseveralautoimmunediseases,includingmultiplesclerosis,psoriasis,juvenilediabetes,rheumatoidarthritis,Crohn"sdiseaseandautoimmuneuveitis.Inaddition,Th17cellsplayanimportantroleintumordevelopment,progressionandmetastasis,potentiallyinbothpromotingandinhibitingtumorgrowth. TheMILLIPLEX?MAPHumanTH17MagneticBeadPanelenablesyoutofocusontherelevanceandtherapeuticpotentialofthemodulationofCD4T-helpercellcytokinesinyourresearchmodel.CoupledwiththeLuminexxMAP?platforminamagneticbeadformat,youreceivetheadvantageofidealspeedandsensitivity,allowingquantitativemultiplexdetectionofmultipleanalytessimultaneously,whichcandramaticallyimproveproductivity.

ProductInformation
Detectionmethod	LuminexxMAP
Configuration	Premixed
Precision,%	Inter-assay:<> Intra-assay:<>
PanelType	MAGNETICCytokines/Chemokines

StorageandShippingInformation
StorageConditions	Recommendedstorageforkitcomponentsis2-8°C.

Applications
Application	SimultaneouslyanalyzemultipleTh17cytokineandchemokinebiomarkerswiththeTh17Bead-BasedMultiplexAssaysusingtheLuminextechnology,inhumanserum,plasmaandcellculturesamples.
KeyApplications	Multiplexing
ApplicationNotes	?Thisisanovernightincubationassayrequiring25μLofneatplasmaorserum,or25μLcellculturesupernatantperwell. ?ThiskitmaybeusedfortheanalysisofalloranycombinationoftheTh17panelanalytesinserum,plasmaortissue/celllysateandculturesupernatant

BIOLOGicalInformation
SpeciesReactivity	Human
AnalytesAvailable	GM-CSF IFN-γ IL-1β IL-2 IL-4 IL-5 IL-6 IL-9 IL-10 IL-12(p70) IL-13 IL-15 IL-17A IL-17F IL-17E/IL-25 IL-21 IL-22 IL-23 IL-27 IL-28A IL-31 IL-33 MIP-3α/CCL20 TNF-α TNFβ
CrossReactivity	Cross-reactivitybetweentheantibodiesandanyoftheotheranalytesinthispanelisnon-detectableornegligIBLe.
Accuracy	79-107%

PhysicochemicalInformation
Sensitivity	Refertokitprotocolforsensitivitiesofindividualbiomarkers.
StandardCurveRange	IL-17&IL-4,24-100,000pg/mL GM-CSF&IL-27,61-250,000pg/mL IFNγ,10-40,000pg/mL IL-10,1-5000pg/mL MIP-3α,IL-1β,IL-12(p70),IL-15,IL-21&IL-33,5-20,000pg/mL IL-13,7-30,000pg/mL IL-17A&IL-2,12-50,000pg/mL IL-22&TNFβ,37-150,000pg/mL IL-9,9-35,000pg/mL IL-23,336-1,500,000pg/mL IL-5,6-25,000pg/mL IL-6&TNFα,3-10,000pg/mL IL-17E/IL-25,488-2,000,000 IL-28a&IL-31,49-200,000pg/mL

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Millipore/HT17MG-14K-PX25 | MILLIPLEX MAP Human TH17 Magnetic Bead Panel Premixed - 25 Plex - Immunology

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