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Millipore/HTS001M | ChemiSCREEN? Membrane Preparation Recombinant Human CXCR1 Chemokine Receptor/HTS001M/200 units

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貨號: HTS001M

品牌: Millipore

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Description
CatalogueNumber	HTS001M
BrandFamily	Chemicon®
TradeName	ChemiScreen Chemicon
Description	ChemiSCREEN?MembranePreparationRecombinantHumanCXCR1ChemokineReceptor
Overview	:Full-lengthhumanCXCR1CDNA
BackgroundInformation	CXCR1(alsoknownasIL-8RA)isaGi/o-coupledGPCRexpressedonneutrophils,CD8(+)Tcells,andintestinalepithelialcells(Murphy,1997;Sturmetal.,2005;Takataetal.,2004).CXCR1bindsspecificallytothechemokineinterleukin-8(IL-8),whereasarelatedreceptor,CXCR2,bindspromiscuouslytoIL-8andseveralotherchemokines.CXCR1hasbeenproposedtobetheprimaryregulatorofneutrophilresponsetoIL-8insepsis(Cummingsetal.,1999).AntagoNISTsofCXCR1andCXCR2havebeendevelopedanddisplayefficacyinanimalmodelsofreperfusioninjury(Bertini,etal.,2004;Kaneideretal.,2005).Millipore"sCXCR1membranepreparationsarecrudemembranepreparationsmadefromourproprietarystablerecombinantcelllinestoensurehigh-levelofGPCRsurfaceexpression;thus,theyareidealHTStoolsforscreeningofantagonistsofCXCR1interactionwithIL-8.ThemembranepreparationsexhibitaKdof0.2nMfor[¹²⁵I]-IL-8.With5μg/wellCXCR1MembranePrepand0.1nM[¹²⁵I]-IL-8,agreaterthan5-foldsignal-to-backgroundratioisobtained.

ProductInformation
Format	Membranes
Presentation	Liquidinpackagingbuffer:50mMTrispH7.4,10%glyceroland1%BSAnopreservatives.Packagingmethod:Membranesproteinwereadjustedtotheindicatedconcentrationinpackagingbuffer,rapidlyfrozen,andstoredat-80°C.

StorageandShippingInformation
StorageConditions	Maintainfrozenat-70°Cforupto2years.Donotfreezeandthaw.

Applications
Application	HumanCXCR1/IL-8aGPCRmembranepreparationforRADIoligandbindingAssays>PgammaSbinding.
KeyApplications	Radioligandbindingassay GTPγSbinding
ApplicationNotes	Radioligandbindingassay,andGTPgammaSbinding.

BIOLOGicalInformation
Concentration	1mg/mL
EntrezGeneNumber	NM_000634.2
EntrezGeneSummary	TheproteinencodedbythisgeneisamemberoftheG-protein-coupledreceptorfamily.Thisproteinisareceptorforinterleukin8(IL8).ItbindstoIL8withhighaffinity,andtransducesthesignalthroughaG-proteinactivatedsecondmessengersystem.Knockoutstudiesinmicesuggestedthatthisproteininhibitsembryonicoligodendrocyteprecursormigrationindevelopingspinalcord.Thisgene,IL8RB,ageneencodinganotherhighaffinityIL8receptor,aswellasIL8RBP,apseudogeneofIL8RB,formageneclusterinaregionmappedtochromosome2q33-q36.
GeneSymbol	IL8RA CMKAR1 CKR-1 C-C C-C-CKR-1 CXCR-1 IL8RBA IL8R1 CDw128a CD128 CXCR1 CD181
GPCRClass	A
HostCells	Chem-1
ProteinTarget	CXCR1/IL-8a
TargetSub-Family	Chemokine
UniProtNumber	P25024
UniProtSummary	FUNCTION:SwissProt:P25024#Receptortointerleukin-8,whichisapowerfulneutrophilschemotacticfactor.BindingofIL-8tothereceptorcausesactivationofneutrophils.ThisresponseismediatedviaaG-proteinthatactivateaphosphatidylinositol-calciumsecondmessengersystem.ThisreceptorbindstoIL-8withahighaffinityandtoMGSA(GRO)withalowaffinity. SIZE:350aminoacids;39791Da SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein. SIMILARITY:SwissProt:P25024##BelongstotheG-proteincoupledreceptor1family.
IncubationConditions	RECOMMENDEDASSAYCONDITIONS:Membranesaremixedwithradioactiveligandandunlabeledcompetitor(seeFigures1and2forconcentrationstested)inbindingbufferinanonbinding96-wellplate,andincubatedfor1-2h.Priortofiltration,anFC96-wellharvestplate(Milliporecat.#MAHFC1H)iscoatedwith0.33%polyethyleneiminefor30min,thenwashedwith50mMHEPES,pH7.4,0.5%BSA.Bindingreactionistransferredtothefilterplate,andwashed3times(1mLperwellperwash)withWashBuffer.Theplateisdriedandcounted. Bindingbuffer:50mMHepes,pH7.4,5mMMgCl₂,1mMCaCl₂,0.2%BSA,filteredandstoredat4°C Radioligand:[¹²⁵I]Interleukin-8(PerkinElmer#NEX277) WashBuffer:50mMHepes,pH7.4,500mMNaCl,0.1%BSA,filteredandstoredat4°C. Onevialcontainsenoughmembranesforatleast200assays(units),whereanunitistheamountofmembranethatwillyieldgreaterthan5-foldsignal:backgroundwith¹²⁵I-labeledIL-8at0.1nM

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Millipore/HTS001M | ChemiSCREEN? Membrane Preparation Recombinant Human CXCR1 Chemokine Receptor/HTS001M/200 units

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