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            當前位置 : Millipore >>> Millipore/HTS002M | ChemiScreen? CXCR2 Membrane Preparation/HTS002M/200 assays
            Millipore/HTS002M | ChemiScreen? CXCR2 Membrane Preparation/HTS002M/200 assays
            • Millipore/HTS002M | ChemiScreen? CXCR2 Membrane Preparation/HTS002M/200 assays

            Millipore/HTS002M | ChemiScreen? CXCR2 Membrane Preparation/HTS002M/200 assays

            價格: ¥7152.00 市場價: 11920.00

            貨號: HTS002M
            品牌: Millipore
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              • Description
                CatalogueNumberHTS002M
                BrandFamilyChemicon®
                TradeName
                • ChemiScreen
                • Chemicon
                DescriptionChemiScreen?CXCR2MembranePreparation
                OverviewTRANSFECTION:Full-lengthhumanCXCR2CDNA(AccessionNumber:M73969)
                BackgroundInformationCXCR2isa7-TMG-proteincoupledreceptorthatbindstothechemokinesGROalpha,GRObeta,GROgamma,IL-8,ENA-78,NAP-2andGCP-2(OlsonandLey,2002).Neutrophils,mastcellsandmicrovascularendothelialcellsexpressCXCR2,andinteractionsofCXCR2withitsligandspromoteschemotaxisofthesecelltypes(Heidemannetal.,2003;Nilssonetal.,1999;Whiteetal.,1998).StudieswithmicelackingCXCR2indicatethatCXCR2promotesgrowthofprimarytumorsandsecondarymetastases(Keaneetal.,2004),andplaysanessentialroleinhyperoxia-inducedlunginjury(Sueetal.,2004).Inaddition,cytomegalovirusencodesaCXCR2-bindingchemokine,vCXC-1,thatpromotesneutrophilmigrationtoinfectedcells(Penfoldetal.,1999).Chemicon"sCXCR2MembranePrepsareidealtoolsforscreeningforantagoNISTsofinteractionsbetweenCXCR2anditsligands.RADIolabeledIL-8bindstotheCXCR2membraneswithaKdof0.075nM.With0.1nM125I-labeledIL-8,a5-foldwindowofspecificbindingisobtained.
                ProductInformation
                FormatMembranes
                PresentationMembraneswereadjustedto0.5mgprotein/mlin50mMTrispH7.4,10%glyceroland1%BSA,rapidlyfrozen,andstoredat-80°C.Onevialtypicallycontains1000units,whereaunitistheamountofmembranethatwillyieldgreaterthan1000cpmspecificbindingof125I-labeledIL-8(0.1nMinputconcentration).
                StorageandShippingInformation
                StorageConditionsMaintainfrozenat-70°Cforupto2years.Donotfreezeandthaw.
                Applications
                ApplicationHumanCXCR2/IL-8bGPCRmembranepreparationforRadioligandbindingAssays.
                KeyApplications
                • Radioligandbindingassay
                ApplicationNotesRadioligandbindingassay.
                BIOLOGicalInformation
                Concentration0.5mg/mL
                EntrezGeneNumber
                EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheG-protein-coupledreceptorfamily.Thisproteinisareceptorforinterleukin8(IL8).ItbindstoIL8withhighaffinity,andtransducesthesignalthroughaG-proteinactivatedsecondmessengersystem.Thisreceptoralsobindstochemokine(C-X-Cmotif)ligand1(CXCL1/MGSA),aproteinwithmelanomagrowthstimulatingactivity,andhasbeenshowntobeamajorcomponentrequiredforserum-dependentmelanomacellgrowth.Thisreceptormediatesneutrophilmigrationtositesofinflammation.TheangiogeniceffectsofIL8inintestinalmicrovascularendothelialcellsarefoundtobemediatedbythisreceptor.Knockoutstudiesinmicesuggestedthatthisreceptorcontrolsthepositioningofoligodendrocyteprecursorsindevelopingspinalcordbyarrestingtheirmigration.Thisgene,IL8RA,ageneencodinganotherhighaffinityIL8receptor,aswellasIL8RBP,apseudogeneofIL8RB,formageneclusterinaregionmappedtochromosome2q33-q36.
                GeneSymbol
                • IL8RB
                • CD182
                • CMKAR2
                • CXCR-2
                • IL8RA
                • IL8R2
                • CXCR2
                • CDw128b
                GPCRClassA
                HostCellsChem-1
                ProteinTargetCXCR2/IL-8b
                TargetSub-FamilyChemokine
                UniProtNumber
                UniProtSummaryFUNCTION:SwissProt:P25025#Receptortointerleukin-8,whichisapowerfulneutrophilschemotacticfactor.BindingofIL-8tothereceptorcausesactivationofneutrophils.ThisresponseismediatedviaaG-proteinthatactivateaphosphatidylinositol-calciumsecondmessengersystem.ThisreceptorbindstoIL-8withahighaffinityandtoGRO/MGSAandNAP-2alsowithahighaffinity.
                SIZE:360aminoacids;40759Da
                SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein.
                PTM:Phosphorylateduponligandbinding;whichisrequiredfordesensitization.
                SIMILARITY:SwissProt:P25025##BelongstotheG-proteincoupledreceptor1family.
                PhysicochemicalInformation
                Dimensions
                MaterialsInformation
                MaterialsInformation
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