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Millipore/IHCR1005-6 | Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1/IHCR1005-6/6 mL

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貨號: IHCR1005-6

品牌: Millipore

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Description
CatalogueNumber	IHCR1005-6
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-TyrosineHydroxylaseAntibody,prediluted,cloneLNC1
BackgroundInformation	Tyrosinehydroxylaseplaysanimportantroleinthephysiologyofadrenergicneurons.Itisthefirstandrate-limitingenzymeinvolvedinthebiosynthesisofthecatecholaminesDopamineandNorepinephrinefromtyrosine.THis,therefore,ausefulMarkerfordopaminergicandnoradrenergicneurons.TheenzymaticactivityofTHrequiresferrousionsascofactorsandisbelievedtoberegulatedbyphosphorylation.AtleastfourisoformsofhumanTHhavebeenidentifiedwhichresultfromalternativesplicing.

ProductInformation
Format	Purified
Presentation	LiquiddilutedinPBS,pH7.2withstABIlizers,0.2%Tween20,and0.1%ProClin300aspreservative.

StorageandShippingInformation
StorageConditions	Maintainat2-8°C.Refertovialforexpirationdating.

Applications
Application	Anti-TyrosineHydroxylaseAntibody,prediluted,cloneLNC1detectslevelofTyrosineHydroxylase&hasbeenpublished&validatedforuseinIH(P).
KeyApplications	Immunohistochemistry(Paraffin)
ApplicationNotes	AntibodyispredilutedandreadytouseforImmunohistochemistryofformalin-fixed,paraffin-embeddedtissues. Pretreatment:HeatInducedEpitopeRetrieval(HIER).RecommendCitrateBuffer,pH6.0(Cat.No.21545).NosignalwasdetectedwithoutEpitoperetrieval. Incubation:30minuteswithIHCSelect?DetectionKits. TyrosineHydroxylasehasbeenpredilutedforuseastheprimaryantibodywithChemicon"sIHCSelect?DetectionKitsandProtocols(CatalogNos.DAB050,DET-HP1000,APR050,andDET-APR1000),butothersupplier"sIHCdetectionsystemsmaybeused.Foroptimizedprotocoldetails,visitwww.chemicon.comandselecttheprotocolslinkunderCat.No.IHCR1005-6.

BIOLOGicalInformation
Immunogen	TyrosineHydroxylasepurifiedfromPC12cells.
Epitope	RecognizesanepitopeontheoutsideoftheregulatoryN-terminusoftyrosinehydroxylase.
Clone	LNC1
Host	Mouse
Specificity	TyrosineHydroxylase.Recognizesaproteinofapproximately59-63kDabyWesternblot.DoesnotreactwiththefollowingonWesternBlots:dopamine-beta-hydroxylase,phenylalaninehydroxylase,trytophanhydroxylase,dehydropteridinereductase,sepiapterinreductase,phenethanolamine-N-methyltransferase(PNMT).
Isotype	IgG1κ
SpeciesReactivity	Chicken Human Mouse Monkey Rat Frog Lizard Vole Zebrafish
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_000360.3 NM_199292.2 NM_199293.2
EntrezGeneSummary	Tyrosinehydroxylaseisinvolvedintheconversionoftyrosinetodopamine.Astherate-limitingenzymeinthesynthesisofcatecholamines,tyrosinehydroxylasehasakeyroleinthephysiologyofadrenergicneurons.
GeneSymbol	TH TYH EC1.14.16.2
UniProtNumber	P07101
UniProtSummary	FUNCTION:SwissProt:P07101#Playsanimportantroleinthephysiologyofadrenergicneurons. COFACTOR:Fe(2+)ion. SIZE:528aminoacids;58524Da TISSUESPECIFICITY:Mainlyexpressedinthebrainandadrenalglands. DISEASE:SwissProt:P07101#DefectsinTHarethecauseofautosomalrecessiveSegawasyndrome[MIM:605407];alsoknownasDOPA-responsivedystonia.Typically,itbeginsinchildhoodoradolescencewithprogressivedifficultyinwalkingand,insomecases,spasticity.Somecasespresentwithparkinsoniansymptomsininfancyandarereferredtoasautosomalrecessiveinfantileparkinsonism. SIMILARITY:SwissProt:P07101##Belongstothebiopterin-dependentaromaticaminoacidhydroxylasefamily.

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Millipore/IHCR1005-6 | Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1/IHCR1005-6/6 mL

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